ArticleJournal of virology2007
Cytomegalovirus UL131-128 products promote gB conformational transition and gB-gH interaction during entry into endothelial cells.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states.Nature communications · 2023Article
- A single, improbable B cell receptor mutation confers potent neutralization against cytomegalovirus.PLoS pathogens · 2023Article
- Prefusion structure of human cytomegalovirus glycoprotein B and structural basis for membrane fusion.Science advances · 2021Article
- Human Cytomegalovirus Enters the Primary CD34Journal of virology · 2019Article
- Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-α as a key for entry.PLoS pathogens · 2017Article
- Palmitoylation Strengthens Cholesterol-dependent Multimerization and Fusion Activity of Human Cytomegalovirus Glycoprotein B (gB).The Journal of biological chemistry · 2016Article
- Human cytomegalovirus tropism for mucosal myeloid dendritic cells.Reviews in medical virology · 2014Review
- Article
- Characterization of a discontinuous neutralizing epitope on glycoprotein B of human cytomegalovirus.Journal of virology · 2013Article
- Glycoprotein B of herpes simplex virus 2 has more than one intracellular conformation and is altered by low pH.Journal of virology · 2012Article
- Glycoprotein N of human cytomegalovirus protects the virus from neutralizing antibodies.PLoS pathogens · 2012Article
- Review
- Protein pUL128 of human cytomegalovirus is necessary for monocyte infection and blocking of migration.Journal of virology · 2011Article
- Mouse embryonic stem cells inhibit murine cytomegalovirus infection through a multi-step process.PloS one · 2011Article
- The glycoprotein B disintegrin-like domain binds beta 1 integrin to mediate cytomegalovirus entry.Journal of virology · 2010Article
- Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication.The Journal of clinical investigation · 2010Article
- Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture.The Journal of general virology · 2010Article
- The m74 gene product of murine cytomegalovirus (MCMV) is a functional homolog of human CMV gO and determines the entry pathway of MCMV.Journal of virology · 2010Article
- Onset of human cytomegalovirus replication in fibroblasts requires the presence of an intact vimentin cytoskeleton.Journal of virology · 2009Article
- Herpes simplex virus gD forms distinct complexes with fusion executors gB and gH/gL in part through the C-terminal profusion domain.The Journal of biological chemistry · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herpesviruses use gB and gH-gL glycoproteins to execute fusion. Other virus-specific glycoproteins are required for receptor binding and fusion activation. The human cytomegalovirus (HCMV) UL131-128 proteins are essential for the infection of leukocytes, endothelial cells (ECs), and many epithelial cell lines. Here we show that UL131-128 play a role in a chain of events involving gB and gH during HCMV entry into ECs. An HCMV strain bearing the wild-type (wt) UL131-128 locus exhibited a gB transition from a protease-resistant to protease-sensitive form, a conformational change that was suppressed by a thiourea inhibitor of fusion (WY1768); in contrast, gH was susceptible to proteolysis throughout entry. Moreover, gB and gH transiently interacted, and a lipid mixing assay showed that the wt strain had carried out fusion by 60 min postinfection. However, these events were greatly altered when UL131-128-defective strains were used for infection or when there was an excess of soluble pUL128 during wt infection: the gB conformational change became WY1768 resistant, the gB-gH complex was no longer observed, and fusion was prevented. Both gB and gH in this case showed late protease resistance, related to their endocytic uptake. Our data point to the involvement of UL131-128 proteins in driving gB through a WY1768-sensitive fold transition, thus promoting a short-lived gB-gH complex and fusion; they also suggest that HCMV fuses with the EC plasma membrane and that endocytosis ensues only when the virus cannot trigger UL131-128-dependent steps.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.