ArticleJournal of virology2007
Rescue of influenza C virus from recombinant DNA.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Simultaneous Packaging of Two Different RNA Segments into an Influenza C Virus-like Particle Occurs Inefficiently.Viruses · 2025Article
- Influenza Reverse Genetics-Historical Perspective.Cold Spring Harbor perspectives in medicine · 2021Review
- Establishment of a Reverse Genetic System from a Bovine Derived Influenza D Virus Isolate.Viruses · 2021Article
- Establishment of a Reverse Genetics System for Influenza D Virus.Journal of virology · 2020Article
- Development and Characterization of a Reverse-Genetics System for Influenza D Virus.Journal of virology · 2019Article
- An Open Receptor-Binding Cavity of Hemagglutinin-Esterase-Fusion Glycoprotein from Newly-Identified Influenza D Virus: Basis for Its Broad Cell Tropism.PLoS pathogens · 2016Article
- Hemagglutinin-esterase-fusion (HEF) protein of influenza C virus.Protein & cell · 2016Review
- RNA virus reverse genetics and vaccine design.Viruses · 2014Review
- Chimeric NP non coding regions between type A and C influenza viruses reveal their role in translation regulation.PloS one · 2014Article
- Molecular basis of a pandemic of avian-type influenza virus.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Many ways to make an influenza virus--review of influenza virus reverse genetics methods.Influenza and other respiratory viruses · 2013Review
- Isolation of a novel swine influenza virus from Oklahoma in 2011 which is distantly related to human influenza C viruses.PLoS pathogens · 2013Article
- The Panhandle formed by influenza A and C virus NS non-coding regions determines NS segment expression.PloS one · 2013Article
- A seven plasmid-based system for the rescue of influenza C virus.Journal of molecular and genetic medicine : an international journal of biomedical research · 2010Article
- Non coding extremities of the seven influenza virus type C vRNA segments: effect on transcription and replication by the type C and type A polymerase complexes.Virology journal · 2008Article
- A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rescue of influenza viruses by reverse genetics has been described only for the influenza A and B viruses. Based on a similar approach, we developed a reverse-genetics system that allows the production of influenza C viruses entirely from cloned cDNA. The complete sequences of the 3' and 5' noncoding regions of type C influenza virus C/Johannesburg/1/66 necessary for the cloning of the cDNA were determined for the seven genomic segments. Human embryonic kidney cells (293T) were transfected simultaneously with seven plasmids that direct the synthesis of each of the seven viral RNA segments of the C/JHB/1/66 virus under the control of the human RNA polymerase I promoter and with four plasmids encoding the viral nucleoprotein and the PB2, PB1, and P3 proteins of the viral polymerase complex. This strategy yielded between 10(3) and 10(4) PFU of virus per ml of supernatant at 8 to 10 days posttransfection. Additional viruses with substitutions introduced in the hemagglutinin-esterase-fusion protein were successfully produced by this method, and their growth phenotype was evaluated. This efficient system, which does not require helper virus infection, should be useful in viral mutagenesis studies and for generation of expression vectors from type C influenza virus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.