ArticleBMC genomics2007
ESTs and EST-linked polymorphisms for genetic mapping and phylogenetic reconstruction in the guppy, Poecilia reticulata.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 24 citations in OpenAlex.
- Divergence and Remarkable Diversity of the Y Chromosome in Guppies.Molecular biology and evolution · 2021Article
- The Genome of the Trinidadian Guppy, Poecilia reticulata, and Variation in the Guanapo Population.PloS one · 2016Article
- Transcriptome assemblies for studying sex-biased gene expression in the guppy, Poecilia reticulata.BMC genomics · 2014Article
- Genomic resources for a model in adaptation and speciation research: characterization of the Poecilia mexicana transcriptome.BMC genomics · 2012Article
- Sequencing and characterization of the guppy (Poecilia reticulata) transcriptome.BMC genomics · 2011Article
- Genetic linkage map of the guppy, Poecilia reticulata, and quantitative trait loci analysis of male size and colour variation.Proceedings. Biological sciences · 2009Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe guppy, Poecilia reticulata, is a well-known model organism for studying inheritance and variation of male ornamental traits as well as adaptation to different river habitats. However, genomic resources for studying this important model were not previously widely available.
resultsWith the aim of generating molecular markers for genetic mapping of the guppy, cDNA libraries were constructed from embryos and different adult organs to generate expressed sequence tags (ESTs). About 18,000 ESTs were annotated according to BLASTN and BLASTX results and the sequence information from the 3' UTRs was exploited to generate PCR primers for re-sequencing of genomic DNA from different wild type strains. By comparison of EST-linked genomic sequences from at least four different ecotypes, about 1,700 polymorphisms were identified, representing about 400 distinct genes. Two interconnected MySQL databases were built to organize the ESTs and markers, respectively. A robust phylogeny of the guppy was reconstructed, based on 10 different nuclear genes.
conclusionOur EST and marker databases provide useful tools for genetic mapping and phylogenetic studies of the guppy.
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