ArticlePLoS biology2007
Precise positioning of myosin VI on endocytic vesicles in vivo.
Article in PLoS biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Direct Observation of Nanometer-Sized Steps of Single Myosin VI Molecules in Living Cells.Nano letters · 2026Article
- Charge engineering controls cooperative assembly and loading in protein host-guest complexes.Journal of materials chemistry. B · 2025Article
- Passive diffusion accounts for the majority of intracellular nanovesicle transport.Life science alliance · 2024Article
- Unconventional Myosins fromBiomolecules · 2022Review
- Time-Resolved Fluorescence Anisotropy and Molecular Dynamics Analysis of a Novel GFP Homo-FRET Dimer.Biophysical journal · 2021Article
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Article
- The Role of Regulatory Domains in Maintaining Autoinhibition in the Multidomain Kinase PKCα.The Journal of biological chemistry · 2017Article
- Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Substrate Affinity Differentially Influences Protein Kinase C Regulation and Inhibitor Potency.The Journal of biological chemistry · 2016Article
- Fluorescent labeling and modification of proteins.Journal of chemical biology · 2013Review
- Membrane-induced lever arm expansion allows myosin VI to walk with large and variable step sizes.The Journal of biological chemistry · 2012Article
- Myosin VI in PC12 cells plays important roles in cell migration and proliferation but not in catecholamine secretion.Journal of muscle research and cell motility · 2011Article
- Imaging the coordination of multiple signalling activities in living cells.Nature reviews. Molecular cell biology · 2011Review
- Principles of unconventional myosin function and targeting.Annual review of cell and developmental biology · 2011Review
- Nucleotide-dependent shape changes in the reverse direction motor, myosin VI.Biophysical journal · 2010Article
- The design of Förster (fluorescence) resonance energy transfer (FRET)-based molecular sensors for Ran GTPase.Methods (San Diego, Calif.) · 2010Review
- Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis.Nature reviews. Molecular cell biology · 2010Review
- Coupled myosin VI motors facilitate unidirectional movement on an F-actin network.The Journal of cell biology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Myosin VI has been studied in both a monomeric and a dimeric form in vitro. Because the functional characteristics of the motor are dramatically different for these two forms, it is important to understand whether myosin VI heavy chains are brought together on endocytic vesicles. We have used fluorescence anisotropy measurements to detect fluorescence resonance energy transfer between identical fluorophores (homoFRET) resulting from myosin VI heavy chains being brought into close proximity. We observed that, when associated with clathrin-mediated endocytic vesicles, myosin VI heavy chains are precisely positioned to bring their tail domains in close proximity. Our data show that on endocytic vesicles, myosin VI heavy chains are brought together in an orientation that previous in vitro studies have shown causes dimerization of the motor. Our results are therefore consistent with vesicle-associated myosin VI existing as a processive dimer, capable of its known trafficking function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.