ArticleMolecular and biochemical parasitology2007
An improved and secreted luciferase reporter for schistosomes.
Article in Molecular and biochemical parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Comprehensive optimization of a reporter assay toolbox for three distinct CRISPR-Cas systems.FEBS open bio · 2021Article
- Transfection of Platyhelminthes.BioMed research international · 2015Review
- Pseudotyped murine leukemia virus for schistosome transgenesis: approaches, methods and perspectives.Transgenic research · 2014Article
- Polyethyleneimine mediated DNA transfection in schistosome parasites and regulation of the WNT signaling pathway by a dominant-negative SmMef2.PLoS neglected tropical diseases · 2013Article
- Molecular characterization of a cytokine-induced apoptosis inhibitor from Schistosoma japonicum.Parasitology research · 2012Article
- Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.PLoS pathogens · 2012Article
- In vivo transfection of developmentally competent Brugia malayi infective larvae.International journal for parasitology · 2011Article
- Molecular characterizations of an inhibitor of apoptosis from Schistosoma japonicum.Parasitology research · 2010Article
- Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferase.Nature medicine · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Schistosomes are multicellular parasites of humans exhibiting interesting biological adaptations to their parasitic lifestyle. Concerted and in depth analyses of these adaptations and their cell and molecular biology requires further development of molecular genetic tools in schistosomes. In the current study, we demonstrate that a Gaussia luciferase reporter leads to significantly higher levels of luciferase activity in schistosomes compared to other tested luciferases. In addition, Gaussia luciferase can be secreted into culture media enabling non-invasive analysis of reporter activity. The secretion of Gaussia luciferase should allow a variety of new experimental paradigms for schistosome studies. Comparison of biolistic and electroporation transfection methods using luciferase RNA reporters and the luciferase acitivty produced indicates that electroporation of sporocysts and schistosomula is the most efficient transfection method for the four stages analyzed. These data should facilitate additional studies in schistosomes and provide a framework for further development of DNA transfection and gene expression analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.