Evidence map›Paper›PMID 17681183›Full record

ArticleGastroenterology2007

MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer.

Fanyin Meng, Roger Henson, Hania Wehbe-Janek, Kalpana Ghoshal, Samson T Jacob, Tushar Patel

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gastroenterology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1,367 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1,367citing papers in PubMed, 6 pooled it
63.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1,367 citing papers in PubMed, 6 syntheses or guidelines pooled it, 2,762 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  4. Pooled it
  5. Pooled it
  6. Pooled it
  7. Exploratory Analysis of PTEN Deficiency by Immunohistochemistry from the Phase III CAPItello-291 Trial.Clinical cancer research : an official journal of the American Association for Cancer Research · 2026
    Trial
  8. Article
  9. Chemically Evolved XNAzyme Platforms for Effective In Vivo Gene Regulation.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Article
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1,307 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Fanyin MengDepartment of Internal Medicine, Scott and White Clinic, Texas A&M University System Health Science Center College of Medicine, Temple, Texas, USA.
Roger Henson
Hania Wehbe-Janek
Kalpana Ghoshal
Samson T Jacob
Tushar Patel
Texas A&M University System · USThe Ohio State University · US

Funding

Mechanisms of Survival Signaling in Biliary EpitheliaR01DK069370 · NIDDK · SCOTT AND WHITE MEMORIAL HOSPITAL · PI PATEL, TUSHAR · 2005 to 2014
$2.4M
The Role of MicroRNA in HepatocarcinogenesisR21CA122694 · NCI · OHIO STATE UNIVERSITY · PI GHOSHAL, KALPANA · 2006 to 2007
$309k
NCI NIH HHS CA122694NCI NIH HHS R21 CA122694NIDDK NIH HHS DK069370NIDDK NIH HHS R01 DK069370
6 · The paper itself

Abstract

BACKGROUND AND

aimsmicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression negatively. Although a role for aberrant miRNA expression in cancer has been postulated, the pathophysiologic role and relevance of aberrantly expressed miRNA to tumor biology has not been established.

methodsWe evaluated the expression of miRNA in human hepatocellular cancer (HCC) by expression profiling, and defined a target gene and biologically functional effect of an up-regulated miRNA.

resultsmiR-21 was noted to be highly overexpressed in HCC tumors and cell lines in expression profiling studies using a miRNA microarray. Inhibition of miR-21 in cultured HCC cells increased expression of the phosphatase and tensin homolog (PTEN) tumor suppressor, and decreased tumor cell proliferation, migration, and invasion. In contrast-enhanced miR-21 expression by transfection with precursor miR-21 increased tumor cell proliferation, migration, and invasion. Moreover, an increase in cell migration was observed in normal human hepatocytes transfected with precursor miR-21. PTEN was shown to be a direct target of miR-21, and to contribute to miR-21 effects on cell invasion. Modulation of miR-21 altered focal adhesion kinase phosphorylation and expression of matrix metalloproteases 2 and 9, both downstream mediators of PTEN involved in cell migration and invasion.

conclusionsAberrant expression of miR-21 can contribute to HCC growth and spread by modulating PTEN expression and PTEN-dependent pathways involved in mediating phenotypic characteristics of cancer cells such as cell growth, migration, and invasion.

Indexed as

Gene Expression Regulation, EnzymologicGene Expression Regulation, NeoplasticCarcinoma, HepatocellularCell Line, TumorCell MovementCell ProliferationFocal Adhesion Protein-Tyrosine KinasesGene Expression ProfilingHumansLiver NeoplasmsMatrix Metalloproteinase 2Matrix Metalloproteinase 9MicroRNAsNeoplasm InvasivenessOligonucleotide Array Sequence AnalysisOligonucleotides, AntisenseFocal Adhesion Protein-Tyrosine KinasesMatrix Metalloproteinase 2Matrix Metalloproteinase 9MicroRNAsMIRN21 microRNA, humanOligonucleotides, AntisensePTEN PhosphohydrolasePTEN protein, humanRNA, MessengerRNA, Small Interfering

Identifiers

PMID17681183
PMCPMC4285346
OpenAlexW2000093333

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.