ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2007
Single-cell A3243G mitochondrial DNA mutation load assays for segregation analysis.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 12 citations in OpenAlex.
- Human amniotic epithelial cells are reprogrammed more efficiently by induced pluripotency than adult fibroblasts.Cellular reprogramming · 2012Article
- Non-random mtDNA segregation patterns indicate a metastable heteroplasmic segregation unit in m.3243A>G cybrid cells.PloS one · 2012Article
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9 authors at 2 institutions in 2 countries.
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Abstract
Segregation of mitochondrial DNA (mtDNA) is an important underlying pathogenic factor in mtDNA mutation accumulation in mitochondrial diseases and aging, but the molecular mechanisms of mtDNA segregation are elusive. Lack of high-throughput single-cell mutation load assays lies at the root of the paucity of studies in which, at the single-cell level, mitotic mtDNA segregation patterns have been analyzed. Here we describe development of a novel fluorescence-based, non-gel PCR restriction fragment length polymorphism method for single-cell A3243G mtDNA mutation load measurement. Results correlated very well with a quantitative in situ Padlock/rolling circle amplification-based genotyping method. In view of the throughput and accuracy of both methods for single-cell A3243G mtDNA mutation load determination, we conclude that they are well suited for segregation analysis.
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