ArticleExperimental cell research2007
Phosphorylation of claudin-4 by PKCepsilon regulates tight junction barrier function in ovarian cancer cells.
Article in Experimental cell research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 91 citations in OpenAlex.
- Ion permeability profiles of renal paracellular channel-forming claudins.Acta physiologica (Oxford, England) · 2025Article
- Expression of the kidney anion exchanger 1 affects WNK4 and SPAK phosphorylation and results in claudin-4 phosphorylation.Heliyon · 2023Article
- The role of claudins in homeostasis.Nature reviews. Nephrology · 2023Review
- Profiling the expression of pro-metastatic genes in association with the clinicopathological features of primary breast cancer.Cancer cell international · 2021Article
- Role of Nuclear Claudin-4 in Renal Cell Carcinoma.International journal of molecular sciences · 2020Article
- Epithelial barrier function properties of the 16HBE14o- human bronchial epithelial cell culture model.Bioscience reports · 2020Article
- Role of Claudin Proteins in Regulating Cancer Stem Cells and Chemoresistance-Potential Implication in Disease Prognosis and Therapy.International journal of molecular sciences · 2019Review
- Temporal Effects of Quercetin on Tight Junction Barrier Properties and Claudin Expression and Localization in MDCK II Cells.International journal of molecular sciences · 2019Article
- CLDN10 promotes a malignant phenotype of osteosarcoma cells via JAK1/Stat1 signaling.Journal of cell communication and signaling · 2019Article
- Increased expression of claudin-12 promotes the metastatic phenotype of human bronchial epithelial cells and is associated with poor prognosis in lung squamous cell carcinoma.Experimental and therapeutic medicine · 2019Article
- Increased expression of claudin-17 promotes a malignant phenotype in hepatocyte via Tyk2/Stat3 signaling and is associated with poor prognosis in patients with hepatocellular carcinoma.Diagnostic pathology · 2018Article
- Differences in the expression profiles of claudin proteins in human gastric carcinoma compared with non‑neoplastic mucosa.Molecular medicine reports · 2018Article
- Differences in the expression profiles of claudin proteins in human nasopharyngeal carcinoma compared with non-neoplastic mucosa.Diagnostic pathology · 2018Article
- Phosphorylation of tight junction transmembrane proteins: Many sites, much to do.Tissue barriers · 2018Review
- Tight Junction Proteins and Signaling Pathways in Cancer and Inflammation: A Functional Crosstalk.Frontiers in physiology · 2018Review
- CLDN2 inhibits the metastasis of osteosarcoma cells via down-regulating the afadin/ERK signaling pathway.Cancer cell international · 2018Article
- Spatial distributions of phosphorylated membrane proteins aquaporin 0 and MP20 across young and aged human lenses.Experimental eye research · 2016Article
- Tight junction between endothelial cells: the interaction between nanoparticles and blood vessels.Beilstein journal of nanotechnology · 2016Review
- PKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels.Molecular biology of the cell · 2015Article
- Changes in the phosphorylation of claudins during the course of experimental colitis.International journal of clinical and experimental pathology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Claudin proteins belong to a large family of transmembrane proteins essential to the formation and maintenance of tight junctions (TJs). In ovarian cancer, TJ protein claudin-4 is frequently overexpressed and may have roles in survival and invasion, but the molecular mechanisms underlying its regulation are poorly understood. In this report, we show that claudin-4 can be phosphorylated by protein kinase C (PKC) at Thr189 and Ser194 in ovarian cancer cells and overexpression of a claudin-4 mutant protein mimicking the phosphorylated state results in the disruption of the barrier function. Furthermore, upon phorbol ester-mediated PKC activation of OVCA433 cells, TJ strength is decreased and claudin-4 localization is altered. Analyses using PKC inhibitors and siRNA suggest that PKCepsilon, an isoform typically expressed in ovarian cancer cells, may be important in the TPA-mediated claudin-4 phosphorylation and weakening of the TJs. Furthermore, immunofluorescence studies showed that claudin-4 and PKCepsilon are co-localized at the TJs in these cells. The modulation of claudin-4 activity by PKCepsilon may not only provide a mechanism for disrupting TJ function in ovarian cancer, but may also be important in the regulation of TJ function in normal epithelial cells.
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