ArticleBMC genomics2007
New pleiotropic effects of eliminating a rare tRNA from Streptomyces coelicolor, revealed by combined proteomic and transcriptomic analysis of liquid cultures.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Intracellular Phage Tail-Like Nanostructures Affect Susceptibility of Streptomyces lividans to Osmotic Stress.mSphere · 2023Article
- A panoramic view of the genomic landscape of the genusMicrobial genomics · 2023Article
- Cytoplasmic contractile injection systems mediate cell death in Streptomyces.Nature microbiology · 2023Article
- A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor.Nature communications · 2023Article
- Article
- Biological Functions and Applications of Virus-Related Bacterial Nanoparticles: A Review.International journal of molecular sciences · 2022Review
- Phage tail-like nanostructures affect microbial interactions between Streptomyces and fungi.Scientific reports · 2021Article
- Review
- Comparative Transcriptome Analysis ofAntibiotics (Basel, Switzerland) · 2019Article
- Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor.Nucleic acids research · 2018Article
- Taxonomy, Physiology, and Natural Products of Actinobacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Systems Biology Approaches to Understand Natural Products Biosynthesis.Frontiers in bioengineering and biotechnology · 2015Review
- Genome-wide analysis of in vivo binding of the master regulator DasR in Streptomyces coelicolor identifies novel non-canonical targets.PloS one · 2015Article
- Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism.BMC microbiology · 2014Article
- Deciphering the regulon of Streptomyces coelicolor AbrC3, a positive response regulator of antibiotic production.Applied and environmental microbiology · 2014Article
- Article
- Evolutionary relationships among actinophages and a putative adaptation for growth in Streptomyces spp.Journal of bacteriology · 2013Article
- In vivo studies suggest that induction of VanS-dependent vancomycin resistance requires binding of the drug to D-Ala-D-Ala termini in the peptidoglycan cell wall.Antimicrobial agents and chemotherapy · 2013Article
- Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundIn Streptomyces coelicolor, bldA encodes the only tRNA for a rare leucine codon, UUA. This tRNA is unnecessary for growth, but is required for some aspects of secondary metabolism and morphological development. We describe a transcriptomic and proteomic analysis of the effects of deleting bldA on cellular processes during submerged culture: conditions relevant to the industrial production of antibiotics.
resultsAt the end of rapid growth, a co-ordinated transient up-regulation of about 100 genes, including many for ribosomal proteins, was seen in the parent strain but not the DeltabldA mutant. Increased basal levels of the signal molecule ppGpp in the mutant strain may be responsible for this difference. Transcripts or proteins from a further 147 genes classified as bldA-influenced were mostly expressed late in culture in the wild-type, though others were significantly transcribed during exponential growth. Some were involved in the biosynthesis of seven secondary metabolites; and some have probable roles in reorganising metabolism after rapid growth. Many of the 147 genes were "function unknown", and may represent unknown aspects of Streptomyces biology. Only two of the 147 genes contain a TTA codon, but some effects of bldA could be traced to TTA codons in regulatory genes or polycistronic operons. Several proteins were affected post-translationally by the bldA deletion. There was a statistically significant but weak positive global correlation between transcript and corresponding protein levels. Different technical limitations of the two approaches were a major cause of discrepancies in the results obtained with them.
conclusionAlthough deletion of bldA has very conspicuous effects on the gross phenotype, the bldA molecular phenotype revealed by the "dualomic" approach has shown that only about 2% of the genome is affected; but this includes many previously unknown effects at a variety of different levels, including post-translational changes in proteins and global cellular physiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.