ArticlePLoS genetics2007
Plasticity of fission yeast CENP-A chromatin driven by relative levels of histone H3 and H4.
Article in PLoS genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 85 citations in OpenAlex.
- Contribution of histone variants to aneuploidy: a cancer perspective.Frontiers in genetics · 2023Pooled it
- Oncohistone H3 E97K mutation facilitates CENP-A mislocalization and chromosomal instability in budding yeast.Nucleic acids research · 2025Article
- Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.Journal of cell science · 2024Article
- Cdc48 and its co-factor Ufd1 extract CENP-A from centromeric chromatin and can induce chromosome elimination in the fission yeast Schizosaccharomyces pombe.Biology open · 2024Article
- Article
- Unraveling Histone Loss in Aging and Senescence.Cells · 2024Review
- Mechanisms of Neutrophil Extracellular Trap Formation and Regulation in Cancers.International journal of molecular sciences · 2023Review
- The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.Nucleic acids research · 2022Article
- Establishment of centromere identity is dependent on nuclear spatial organization.Current biology : CB · 2022Article
- Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Epigenetic dynamics of centromeres and neocentromeres in Cryptococcus deuterogattii.PLoS genetics · 2021Article
- Hap2-Ino80-facilitated transcription promotes de novo establishment of CENP-A chromatin.Genes & development · 2020Article
- Histone stress: an unexplored source of chromosomal instability in cancer?Current genetics · 2019Review
- Interspecies conservation of organisation and function between nonhomologous regional centromeres.Nature communications · 2019Article
- Centromeric and ectopic assembly of CENP-A chromatin in health and cancer: old marks and new tracks.Nucleic acids research · 2019Review
- Centromere DNA Destabilizes H3 Nucleosomes to Promote CENP-A Deposition during the Cell Cycle.Current biology : CB · 2018Article
- Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution inThe Journal of biological chemistry · 2018Article
- Article
- Prolyl isomerization of the CENP-A N-terminus regulates centromeric integrity in fission yeast.Nucleic acids research · 2018Article
- The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.The Journal of cell biology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The histone H3 variant CENP-A assembles into chromatin exclusively at centromeres. The process of CENP-A chromatin assembly is epigenetically regulated. Fission yeast centromeres are composed of a central kinetochore domain on which CENP-A chromatin is assembled, and this is flanked by heterochromatin. Marker genes are silenced when placed within kinetochore or heterochromatin domains. It is not known if fission yeast CENP-A(Cnp1) chromatin is confined to specific sequences or whether histone H3 is actively excluded. Here, we show that fission yeast CENP-A(Cnp1) can assemble on noncentromeric DNA when it is inserted within the central kinetochore domain, suggesting that in fission yeast CENP-A(Cnp1) chromatin assembly is driven by the context of a sequence rather than the underlying DNA sequence itself. Silencing in the central domain is correlated with the amount of CENP-A(Cnp1) associated with the marker gene and is also affected by the relative level of histone H3. Our analyses indicate that kinetochore integrity is dependent on maintaining the normal ratio of H3 and H4. Excess H3 competes with CENP-A(Cnp1) for assembly into central domain chromatin, resulting in less CENP-A(Cnp1) and other kinetochore proteins at centromeres causing defective kinetochore function, which is manifest as aberrant mitotic chromosome segregation. Alterations in the levels of H3 relative to H4 and CENP-A(Cnp1) influence the extent of DNA at centromeres that is packaged in CENP-A(Cnp1) chromatin and the composition of this chromatin. Thus, CENP-A(Cnp1) chromatin assembly in fission yeast exhibits plasticity with respect to the underlying sequences and is sensitive to the levels of CENP-A(Cnp1) and other core histones.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.