ReviewBiochemistry2007
Two-photon microscopy: shedding light on the chemistry of vision.
Review in Biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Cracking the Skin Barrier: Models and Methods Driving Dermal Drug Delivery.Pharmaceutics · 2025Review
- Quantification of Photoreceptors' Changes in a Diabetic Retinopathy Model with Two-Photon Imaging Microscopy.International journal of molecular sciences · 2024Article
- Two-Photon and Multiphoton Microscopy in Anterior Segment Diseases of the Eye.International journal of molecular sciences · 2024Review
- Arrangement of the photoreceptor mosaic in a diabetic rat model imaged with multiphoton microscopy.Biomedical optics express · 2020Article
- Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes.Chemical science · 2017Article
- Synthesis of Near-Infrared Fluorescent Two-Photon-Absorbing Fluorenyl Benzothiadiazole and Benzoselenadiazole Derivatives.ACS omega · 2016Article
- Adaptive optics scanning laser ophthalmoscope imaging: technology update.Clinical ophthalmology (Auckland, N.Z.) · 2016Review
- Applications of phototransformable fluorescent proteins for tracking the dynamics of cellular components.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Review
- Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy.Biomedical optics express · 2014Article
- Chemistry of the retinoid (visual) cycle.Chemical reviews · 2014Review
- In vivo two-photon imaging of the mouse retina.Biomedical optics express · 2013Article
- Label-free spatial analysis of free and enzyme-bound NAD(P)H in the presence of high concentrations of melanin.Journal of fluorescence · 2012Article
- Histopathology and functional correlations in a patient with a mutation in RPE65, the gene for retinol isomerase.Investigative ophthalmology & visual science · 2011Article
- Preparation of living isolated vertebrate photoreceptor cells for fluorescence imaging.Journal of visualized experiments : JoVE · 2011Article
- Two-photon excited autofluorescence imaging of freshly isolated frog retinas.Biomedical optics express · 2011Article
- Noninvasive multiphoton fluorescence microscopy resolves retinol and retinal condensation products in mouse eyes.Nature medicine · 2010Article
- Going deeper than microscopy: the optical imaging frontier in biology.Nature methods · 2010Review
- 2-Hydroxypropyl-beta-cyclodextrin removes all-trans retinol from frog rod photoreceptors in a concentration-dependent manner.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2010Article
- Visualization of retinoid storage and trafficking by two-photon microscopy.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Measurement of the mobility of all-trans-retinol with two-photon fluorescence recovery after photobleaching.Methods in molecular biology (Clifton, N.J.) · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Two-photon microscopy (TPM) has come to occupy a prominent place in modern biological research with its ability to resolve the three-dimensional distribution of molecules deep inside living tissue. TPM can employ two different types of signals, fluorescence and second harmonic generation, to image biological structures with subcellular resolution. Two-photon excited fluorescence imaging is a powerful technique with which to monitor the dynamic behavior of the chemical components of tissues, whereas second harmonic imaging provides novel ways to study their spatial organization. Using TPM, great strides have been made toward understanding the metabolism, structure, signal transduction, and signal transmission in the eye. These include the characterization of the spatial distribution, transport, and metabolism of the endogenous retinoids, molecules essential for the detection of light, as well as the elucidation of the architecture of the living cornea. In this review, we present and discuss the current applications of TPM for the chemical and structural imaging of the eye. In addition, we address what we see as the future potential of TPM for eye research. This relatively new method of microscopy has been the subject of numerous technical improvements in terms of the optics and indicators used, improvements that should lead to more detailed biochemical characterizations of the eyes of live animals and even to imaging of the human eye in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.