ArticleNature methods2007
An HSV vector system for selection of ligand-gated ion channel modulators.
Article in Nature methods, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Molecular Mechanisms of Neurogenic Lower Urinary Tract Dysfunction after Spinal Cord Injury.International journal of molecular sciences · 2023Review
- An HSV-based library screen identifies PP1α as a negative TRPV1 regulator with analgesic activity in models of pain.Molecular therapy. Methods & clinical development · 2016Article
- Herpes Simplex Virus Vector-Mediated Gene Delivery of Poreless TRPV1 Channels Reduces Bladder Overactivity and Nociception in Rats.Human gene therapy · 2015Article
- Relief of pain induced by varicella-zoster virus in a rat model of post-herpetic neuralgia using a herpes simplex virus vector expressing enkephalin.Gene therapy · 2014Article
- Herpes simplex viral vectors: late bloomers with big potential.Human gene therapy · 2014Review
- Effects of herpes simplex virus vector-mediated enkephalin gene therapy on bladder overactivity and nociception.Human gene therapy · 2013Article
- Gene therapy for the treatment of chronic peripheral nervous system pain.Neurobiology of disease · 2012Review
- A herpes simplex virus vector system for expression of complex cellular cDNA libraries.Journal of virology · 2010Article
- Recombinant adeno-associated virus serotype 6 (rAAV2/6)-mediated gene transfer to nociceptive neurons through different routes of delivery.Molecular pain · 2009Article
- HSV vector-mediated modification of primary nociceptor afferents: an approach to inhibit chronic pain.Gene therapy · 2009Review
- Herpes vector-mediated gene transfer in the treatment of chronic pain.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Protein kinase C epsilon contributes to basal and sensitizing responses of TRPV1 to capsaicin in rat dorsal root ganglion neurons.The European journal of neuroscience · 2008Article
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10 authors at 1 institution in 1 country.
Funding
Abstract
Pathological alterations of ion channel activity result from changes in modulatory mechanisms governing receptor biology. Here we describe a conditional herpes simplex virus (HSV) replication-based strategy to discover channel modulators whereby inhibition of agonist-induced channel activation by a vector-expressed modulatory gene product prevents ion flux, osmotic shock and cell death. Inhibition of channel activity, in this case, the rat vanilloid (Trpv1 or the glycine receptor (GlyRalpha1), can allow selection of escape vector plaques containing the 'captured' modulatory gene for subsequent identification and functional analysis. We validated this prediction using mixed infections of a wild-type Trpv1 expression vector vTTHR and a nonfunctional 'poreless' Trpv1 subunit-expressing vector, vHP, wherein vHP was highly selected from a large background of vTTHR viruses in the presence of the Trpv1 agonist, capsaicin. The approach should be useful for probing large libraries of vector-expressed cDNAs for the presence of ion channel modulators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.