ArticleApplied and environmental microbiology2007
Characterization of protein-protein interactions involved in iron reduction by Shewanella oneidensis MR-1.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 164 citations in OpenAlex.
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- Microbe-Anode Interactions: Comparing the impact of genetic and material engineering approaches to improve the performance of microbial electrochemical systems (MES).Microbial biotechnology · 2023Review
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- Single molecule tracking of bacterial cell surface cytochromes reveals dynamics that impact long-distance electron transport.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Extracellular Electron Transfer Enables Cellular Control of Cu(I)-Catalyzed Alkyne-Azide Cycloaddition.ACS central science · 2022Article
- Pyruvate accelerates palladium reduction by regulating catabolism and electron transfer pathway inApplied and environmental microbiology · 2021Article
- Engineering S. oneidensis for Performance Improvement of Microbial Fuel Cell-a Mini Review.Applied biochemistry and biotechnology · 2021Review
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- Tuning Extracellular Electron Transfer byACS synthetic biology · 2020Article
- A Hybrid Extracellular Electron Transfer Pathway Enhances the Survival of Vibrio natriegens.Applied and environmental microbiology · 2020Article
- Biological synthesis of iron nanoparticles using hydrolysates from a waste-based biorefinery.Environmental science and pollution research international · 2020Article
- Metal Reduction and Protein Secretion Genes Required for Iodate Reduction by Shewanella oneidensis.Applied and environmental microbiology · 2019Article
- Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and COMicrobial cell factories · 2019Article
- Goethite Hinders Azo Dye Bioreduction by Blocking Terminal Reductive Sites on the Outer Membrane ofFrontiers in microbiology · 2019Article
- Divergent Nrf Family Proteins and MtrCAB Homologs Facilitate Extracellular Electron Transfer in Aeromonas hydrophila.Applied and environmental microbiology · 2018Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The interaction of proteins implicated in dissimilatory metal reduction by Shewanella oneidensis MR-1 (outer membrane [OM] proteins OmcA, MtrB, and MtrC; OM-associated protein MtrA; periplasmic protein CctA; and cytoplasmic membrane protein CymA) were characterized by protein purification, analytical ultracentrifugation, and cross-linking methods. Five of these proteins are heme proteins, OmcA (83 kDa), MtrC (75 kDa), MtrA (32 kDa), CctA (19 kDa), and CymA (21 kDa), and can be visualized after sodium dodecyl sulfate-polyacrylamide gel electrophoresis by heme staining. We show for the first time that MtrC, MtrA, and MtrB form a 198-kDa complex with a 1:1:1 stoichiometry. These proteins copurify through anion-exchange chromatography, and the purified complex has the ability to reduce multiple forms of Fe(III) and Mn(IV). Additionally, MtrA fractionates with the OM through sucrose density gradient ultracentrifugation, and MtrA comigrates with MtrB in native gels. Protein cross-linking of whole cells with 1% formaldehyde show new heme bands of 160, 151, 136, and 59 kDa. Using antibodies to detect each protein separately, heme proteins OmcA and MtrC were shown to cross-link, yielding the 160-kDa band. Consistent with copurification results, MtrB cross-links with MtrA, forming high-molecular-mass bands of approximately 151 and 136 kDa.
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Registered trials
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