ArticleApplied and environmental microbiology2007
Directed evolution of Vibrio fischeri LuxR for improved response to butanoyl-homoserine lactone.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Biological Switches: Past and Future Milestones of Transcription Factor-Based Biosensors.ACS synthetic biology · 2025Review
- Directed Evolution of 4-Hydroxyphenylpyruvate Biosensors Based on a Dual Selection System.International journal of molecular sciences · 2024Article
- Cross-species activation of hydrogen cyanide production by a promiscuous quorum-sensing receptor promotesMicrobiology (Reading, England) · 2023Article
- Natural and synthetic inhibitors of a phage-encoded quorum-sensing receptor affect phage-host dynamics in mixed bacterial communities.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Repurposing phytochemicals as anti-virulent agents to attenuate quorum sensing-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa.Microbial biotechnology · 2022Review
- A plasmid toolbox for controlled gene expression across the Proteobacteria.Nucleic acids research · 2021Article
- Winner-takes-all resource competition redirects cascading cell fate transitions.Nature communications · 2021Article
- Computer-Aided Rational Engineering of Signal Sensitivity of Quorum Sensing Protein LuxR in a Whole-Cell Biosensor.Frontiers in molecular biosciences · 2021Article
- Transcription Factor Engineering for High-Throughput Strain Evolution and Organic Acid Bioproduction: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Evidence of a Large Diversity of N-acyl-Homoserine Lactones in Symbiotic Vibrio fischeri Strains Associated with the Squid Euprymna scolopes.Microbes and environments · 2019Article
- Phosphatase activity tunes two-component system sensor detection threshold.Nature communications · 2018Article
- Tailor-made transcriptional biosensors for optimizing microbial cell factories.Journal of industrial microbiology & biotechnology · 2017Review
- Optimization of industrial microorganisms: recent advances in synthetic dynamic regulators.Journal of industrial microbiology & biotechnology · 2017Review
- Specificity and complexity in bacterial quorum-sensing systems.FEMS microbiology reviews · 2016Review
- Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems.Metabolic engineering · 2015Review
- In Vivo Programmed Gene Expression Based on Artificial Quorum Networks.Applied and environmental microbiology · 2015Article
- Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities.Nucleic acids research · 2015Article
- Modeling Analysis of Signal Sensitivity and Specificity by Vibrio fischeri LuxR Variants.PloS one · 2015Article
- Directed evolution as a powerful synthetic biology tool.Methods (San Diego, Calif.) · 2013Article
- Transcription factor-based screens and synthetic selections for microbial small-molecule biosynthesis.ACS synthetic biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LuxR is the 3-oxohexanoyl-homoserine lactone (3OC6HSL)-dependent transcriptional activator of the prototypical acyl-homoserine lactone (AHL) quorum-sensing system of Vibrio fischeri. Wild-type LuxR exhibits no response to butanoyl-HSL (C4HSL) in quantitative bioassays at concentrations of up to 1 microM; a previously described LuxR variant (LuxR-G2E) exhibits a broadened response to diverse AHLs, including pentanoyl-HSL (C5HSL), but not to C4HSL. Here, two rounds of directed evolution of LuxR-G2E generated variants of LuxR that responded to C4HSL at concentrations as low as 10 nM. One variant, LuxR-G4E, had only one change, I45F, relative to the parent LuxR-G2E, which itself differs from the wild type at three residues. Dissection of the four mutations within LuxR-G4E demonstrated that at least three of these changes were simultaneously required to achieve any measurable C4HSL response. The four changes improved both sensitivity and specificity towards C4HSL relative to any of the other 14 possible combinations of those residues. These data confirm that LuxR is evolutionarily pliable and suggest that LuxR is not intrinsically asymmetric in its response to quorum-sensing signals with different acyl-side-chain lengths.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.