ArticleThe EMBO journal2007
Structural and functional characterization of a novel type of ligand-independent RXR-USP receptor.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 129 citations in OpenAlex.
- Retinoid-X receptor action and disruption in development.Developmental biology · 2026Review
- A multidisciplinary approach disclosing unexplored Aflatoxin B1 roles in severe impairment of vitamin D mechanisms of action.Cell biology and toxicology · 2023Article
- In Vitro Binding Effects of the Ecdysone Receptor-Binding Domain and PonA inMolecules (Basel, Switzerland) · 2023Article
- Molecular interplay between ecdysone receptor and retinoid X receptor in regulating the molting of the Chinese mitten crab,Frontiers in endocrinology · 2023Article
- From Extrapolation to Precision Chemical Hazard Assessment: The Ecdysone Receptor Case Study.Toxics · 2021Article
- A structural signature motif enlightens the origin and diversification of nuclear receptors.PLoS genetics · 2021Article
- Nonsteroidal ecdysone receptor agonists use a water channel for binding to the ecdysone receptor complex EcR/USP.Journal of pesticide science · 2021Article
- Review
- Of Retinoids and Organotins: The Evolution of the Retinoid X Receptor in Metazoa.Biomolecules · 2020Article
- Genes Encoding Teleost Fish Ligands and Associated Receptors Remained in Duplicate More Frequently than the Rest of the Genome.Genome biology and evolution · 2019Article
- Conservation of DNA and ligand binding properties of retinoid X receptor from the placozoan Trichoplax adhaerens to human.The Journal of steroid biochemistry and molecular biology · 2018Review
- Article
- Exploration of the binding affinities between ecdysone agonists and EcR/USP by docking and MM-PB/GBSA approaches.Journal of molecular modeling · 2017Article
- The retinoid X receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.Scientific reports · 2016Article
- 9-cis-13,14-Dihydroretinoic Acid Is an Endogenous Retinoid Acting as RXR Ligand in Mice.PLoS genetics · 2015Article
- Cloning, ligand-binding, and temporal expression of ecdysteroid receptors in the diamondback moth, Plutella xylostella.BMC molecular biology · 2012Article
- Molecular adaptation and resilience of the insect's nuclear receptor USP.BMC evolutionary biology · 2012Article
- Sequencing and structural homology modeling of the ecdysone receptor in two chrysopids used in biological control of pest insects.Ecotoxicology (London, England) · 2012Article
- The retinoid X receptors and their ligands.Biochimica et biophysica acta · 2012Review
- Crystal structure of Fushi tarazu factor 1 ligand binding domain/Fushi tarazu peptide complex identifies new class of nuclear receptors.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retinoid X receptor (RXR) and Ultraspiracle (USP) play a central role as ubiquitous heterodimerization partners of many nuclear receptors. While it has long been accepted that a wide range of ligands can activate vertebrate/mollusc RXRs, the existence and necessity of specific endogenous ligands activating RXR-USP in vivo is still matter of intense debate. Here we report the existence of a novel type of RXR-USP with a ligand-independent functional conformation. Our studies involved Tribolium USP (TcUSP) as representative of most arthropod RXR-USPs, with high sequence homology to vertebrate/mollusc RXRs. The crystal structure of the ligand-binding domain of TcUSP was solved in the context of the functional heterodimer with the ecdysone receptor (EcR). While EcR exhibits a canonical ligand-bound conformation, USP adopts an original apo structure. Our functional data demonstrate that TcUSP is a constitutively silent partner of EcR, and that none of the RXR ligands can bind and activate TcUSP. These findings together with a phylogenetic analysis suggest that RXR-USPs have undergone remarkable functional shifts during evolution and give insight into receptor-ligand binding evolution and dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.