ArticleBMC plant biology2007
An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library.
Article in BMC plant biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 67 citations in OpenAlex.
- Expression Profiles, Characterization and Function of HbTCTP in Rubber Tree (Hevea brasiliensis).Frontiers in plant science · 2016Article
- Gene Structures, Evolution, Classification and Expression Profiles of the Aquaporin Gene Family in Castor Bean (Ricinus communis L.).PloS one · 2015Article
- De novo transcriptome sequence assembly from coconut leaves and seeds with a focus on factors involved in RNA-directed DNA methylation.G3 (Bethesda, Md.) · 2014Article
- Structural divergence of plant TCTPs.Frontiers in plant science · 2014Article
- Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development.Genetics and molecular biology · 2013Article
- Homologous electron transport components fail to increase fatty acid hydroxylation in transgenic Arabidopsis thaliana.F1000Research · 2013Article
- Construction and evaluation of normalized cDNA libraries enriched with full-length sequences for rapid discovery of new genes from Sisal (Agave sisalana Perr.) different developmental stages.International journal of molecular sciences · 2012Article
- The phosphatidylcholine diacylglycerol cholinephosphotransferase is required for efficient hydroxy fatty acid accumulation in transgenic Arabidopsis.Plant physiology · 2012Article
- Global analysis of gene expression profiles in developing physic nut (Jatropha curcas L.) seeds.PloS one · 2012Article
- Tissue-specific whole transcriptome sequencing in castor, directed at understanding triacylglycerol lipid biosynthetic pathways.PloS one · 2012Article
- Comparative deep transcriptional profiling of four developing oilseeds.The Plant journal : for cell and molecular biology · 2011Article
- Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis.Molecular biology reports · 2011Article
- Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.).BMC plant biology · 2010Article
- Mining the bitter melon (momordica charantia l.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes.BMC plant biology · 2010Article
- Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds.BMC genomics · 2010Article
- Transcriptome analysis of the oil-rich seed of the bioenergy crop Jatropha curcas L.BMC genomics · 2010Article
- Probing the endosperm gene expression landscape in Brassica napus.BMC genomics · 2009Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCastor seeds are a major source for ricinoleate, an important industrial raw material. Genomics studies of castor plant will provide critical information for understanding seed metabolism, for effectively engineering ricinoleate production in transgenic oilseeds, or for genetically improving castor plants by eliminating toxic and allergic proteins in seeds.
resultsFull-length cDNAs are useful resources in annotating genes and in providing functional analysis of genes and their products. We constructed a full-length cDNA library from developing castor endosperm, and obtained 4,720 ESTs from 5'-ends of the cDNA clones representing 1,908 unique sequences. The most abundant transcripts are genes encoding storage proteins, ricin, agglutinin and oleosins. Several other sequences are also very numerous, including two acidic triacylglycerol lipases, and the oleate hydroxylase (FAH12) gene that is responsible for ricinoleate biosynthesis. The role(s) of the lipases in developing castor seeds are not clear, and co-expressing of a lipase and the FAH12 did not result in significant changes in hydroxy fatty acid accumulation in transgenic Arabidopsis seeds. Only one oleate desaturase (FAD2) gene was identified in our cDNA sequences. Sequence and functional analyses of the castor FAD2 were carried out since it had not been characterized previously. Overexpression of castor FAD2 in a FAH12-expressing Arabidopsis line resulted in decreased accumulation of hydroxy fatty acids in transgenic seeds.
conclusionOur results suggest that transcriptional regulation of FAD2 and FAH12 genes maybe one of the mechanisms that contribute to a high level of ricinoleate accumulation in castor endosperm. The full-length cDNA library will be used to search for additional genes that affect ricinoleate accumulation in seed oils. Our EST sequences will also be useful to annotate the castor genome, which whole sequence is being generated by shotgun sequencing at the Institute for Genome Research (TIGR).
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