ArticlePlanta2007
Laser capture microdissection (LCM) and comparative microarray expression analysis of syncytial cells isolated from incompatible and compatible soybean (Glycine max) roots infected by the soybean cyst nematode (Heterodera glycines).
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 169 citations in OpenAlex.
- Genome Identification ofPlants (Basel, Switzerland) · 2026Article
- Research progress on the functional study of host resistance-related genes against Heterodera glycines.Crop health · 2023Review
- Identification of QTL, QTL-by-environment interactions, and their candidate genes for resistance HG Type 0 and HG Type 1.2.3.5.7 in soybean using 3VmrMLM.Frontiers in plant science · 2023Article
- The heterologous expression of conserved Glycine max (soybean) mitogen activated protein kinase 3 (MAPK3) paralogs suppresses Meloidogyne incognita parasitism in Gossypium hirsutum (upland cotton).Transgenic research · 2022Article
- Regulation of heat shock proteins 70 and their role in plant immunity.Journal of experimental botany · 2022Review
- Kinase-dead mutation: A novel strategy for improving soybean resistance to soybean cyst nematode Heterodera glycines.Molecular plant pathology · 2022Article
- A Broad Review of Soybean Research on the Ongoing Race to Overcome Soybean Cyst Nematode.Biology · 2022Review
- Comparisons of constitutive resistances to soybean cyst nematode between PI 88788- and Peking-type sources of resistance in soybean by transcriptomic and metabolomic profilings.Frontiers in genetics · 2022Article
- Weighted gene co-expression network analysis identifies genes related to HG Type 0 resistance and verification of hub geneFrontiers in plant science · 2022Article
- Article
- RNA Isolation from Nematode-Induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Omics Hunt for Novel Molecular Markers of Resistance toPlants (Basel, Switzerland) · 2021Article
- Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots.International journal of molecular sciences · 2021Article
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- Article
- Article
- Early transcriptional responses to soybean cyst nematode HG Type 0 show genetic differences among resistant and susceptible soybeans.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- A MIF-like effector suppresses plant immunity and facilitates nematode parasitism by interacting with plant annexins.Journal of experimental botany · 2019Article
- Transcriptional profiling of wheat (Triticum aestivum L.) during a compatible interaction with the cereal cyst nematode Heterodera avenae.Scientific reports · 2019Article
- Genomics of Plant Disease Resistance in Legumes.Frontiers in plant science · 2019Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Syncytial cells in soybean (Glycine max cultivar [cv.] Peking) roots infected by incompatible and compatible populations of soybean cyst nematode (SCN [Heterodera glycines]) were collected using laser capture microdissection (LCM). Gene transcript abundance was assayed using Affymetrix soybean GeneChips, each containing 37,744 probe sets. Our analyses identified differentially expressed genes in syncytial cells that are not differentially expressed in the whole root analyses. Therefore, our results show that the mass of transcriptional activity occurring in the whole root is obscuring identification of transcriptional events occurring within syncytial cells. In syncytial cells from incompatible roots at three dpi, genes encoding lipoxygenase (LOX), heat shock protein (HSP) 70, superoxidase dismutase (SOD) were elevated almost tenfold or more, while genes encoding several transcription factors and DNA binding proteins were also elevated, albeit at lower levels. In syncytial cells formed during the compatible interaction at three dpi, genes encoding prohibitin, the epsilon chain of ATP synthase, allene oxide cyclase and annexin were more abundant. By 8 days, several genes of unknown function and genes encoding a germin-like protein, peroxidase, LOX, GAPDH, 3-deoxy-D-arabino-heptolosonate 7-phosphate synthase, ATP synthase and a thioesterase were abundantly expressed. These observations suggest that gene expression is different in syncytial cells as compared to whole roots infected with nematodes. Our observations also show that gene expression is different between syncytial cells that were isolated from incompatible and compatible roots and that gene expression is changing over the course of syncytial cell development as it matures into a functional feeding site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.