ArticlePflugers Archiv : European journal of physiology2007
Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells.
Article in Pflugers Archiv : European journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
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- Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.Cells · 2020Article
- Article
- Mesenchymal stem cells confer chemoresistance in breast cancer via a CD9 dependent mechanism.Oncotarget · 2019Article
- Altered properties of feline adipose-derived mesenchymal stem cells during continuous in vitro cultivation.The Journal of veterinary medical science · 2018Article
- Cancer stem cell surface markers on normal stem cells.BMB reports · 2017Review
- Mesenchymal stem/stromal cells-a key mediator for regeneration after perinatal morbidity?Molecular and cellular pediatrics · 2016Article
- Therapeutic doses of multipotent stromal cells from minimal adipose tissue.Stem cell reviews and reports · 2014Article
- Role of motility-related protein-1 in promoting the development of several types of cancer (Review).Oncology letters · 2014Article
- Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation.Clinics (Sao Paulo, Brazil) · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD9 belongs to the tetraspanin family and is involved in cell motility, osteoclastogenesis, metastasis, neurite outgrowth, myotube formation, and sperm-egg fusion. CD9 also promotes juxtacrine signaling involved in proliferation and attachment. Varying degrees of CD9 expression have been found in human mesenchymal stem cells. In this study, we determined the functional roles of CD9 in human adipose-derived mesenchymal stem cells (hASCs). The CD9 expression in hASCs was down-regulated during culture expansion. A colony-forming unit assay revealed that the clonal expandability of hASCs was directly correlated with the CD9 expression level of the colony. The CD9(high) cells exhibited an increased ability to proliferate, increased cell adhesiveness, and better in vitro tube formation than the CD9(low) cells. The cellular proliferation and attachment of the CD9(high) cells were inhibited upon treatment with a blocking antibody against CD9 and the transduction of a CD9 miRNA lentivirus. The CD9(high) cells showed higher NF-kappaB promoter activity and higher levels of intercellular adhesion molecule 1 than the CD9(low) cells. Reverse transcription-polymerase chain reaction analysis revealed higher endothelial nitric oxide synthase expression in the CD9(high) cells than in the CD9(low) cells. The engraftment and the proangiogenic action of hASCs in a murine model of hindlimb ischemia were significantly higher in the CD9(high) cells than in the CD9(low) cells. This study indicates that CD9 plays roles in cell proliferation and attachment in vitro as well as in in vivo engraftment and that it can be considered as a useful marker to predict the in vivo efficacy of hASCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.