ArticlePlant cell reports2007
Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.).
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 126 citations in OpenAlex.
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- Tissue Culture-A Sustainable Approach to Explore Plant Stresses.Life (Basel, Switzerland) · 2023Review
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- Genomics Enabled Breeding Strategies for Major Biotic Stresses in Pea (Frontiers in plant science · 2022Review
- Agronomical, Physiological and Biochemical Characterization of In Vitro Selected Eggplant Somaclonal Variants under NaCl Stress.Plants (Basel, Switzerland) · 2021Article
- Study of the amount of oxidative damage to mitochondrial and chloroplast DNA in clones of white poplar (Populus alba L.) during long-term in vitro cultivation for 26 years.Plant molecular biology · 2021Article
- Transcriptome analysis of differentially expressed genes in chrysanthemumPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Tissue culture-induced DNA methylation in crop plants: a review.Molecular biology reports · 2021Review
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- Efficient Tissue Culture Protocol forPlants (Basel, Switzerland) · 2020Article
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- Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example.Plants (Basel, Switzerland) · 2020Article
- Systematic Analysis of the DNA Methylase and Demethylase Gene Families in Rapeseed (International journal of molecular sciences · 2020Article
- Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers.Plant cell reports · 2018Article
- Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process.Plant cell reports · 2016Article
- DNA methylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.).Journal of biological research (Thessalonike, Greece) · 2016Article
- Effect of Cryopreservation and Post-Cryopreservation Somatic Embryogenesis on the Epigenetic Fidelity of Cocoa (Theobroma cacao L.).PloS one · 2016Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro clonal propagation of plants should generate identical copies of the selected genotype. However, associated stress might result in a breakdown of control mechanisms and consequent instability of the genome. We have used several molecular methods to assess the genetic stability of long-term propagated (24 years) multiple shoot in vitro culture of pea (Pisum sativum L.). We focused on assessing the stability of repetitive sequences, such as simple sequence repeats (SSR) and retrotransposons, both comprising a large part of genome. No differences were found when seedlings (Co-2004) or original seed (Co-1982) controls and long-term or newly established in vitro (one subculture cycle) samples were investigated by the SSR, inter-repeats (ISSR) or inter-retrotransposon amplified polymorphism (IRAP) method. However, the more global amplified fragment length polymorphism (AFLP) and particularly the methylation sensitive MSAP methods detected 11 and 18% polymorphism among samples, respectively. Interestingly, investigation of the global cytosine methylation status by HPCE measurement revealed no statistically significant differences. Some evidence of retrotransposon re-arrangement was observed by sequence-specific amplification polymorphism. This occurred mostly in the abundant Ty3-gypsy type Cyclop element and to a smaller extent in the Ogre element. Alternatively, no polymorphism was detected among the PDR-1 element of the Ty1-copia type retrotransposon. Based on these results, multiple shoot culture of pea maintained over a long period may be considered as a true to type multiplication method of the original genotype.
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