ArticleMolecular therapy : the journal of the American Society of Gene Therapy2007
Intranuclear trafficking of episomal DNA is transcription-dependent.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Functional role of Galectin-9 in nucleic acid trafficking and transcription post-electrotransfection.NAR molecular medicine · 2026Article
- Subnuclear organization and mislocalization of plasmids reduce transgene expression.Molecular therapy. Nucleic acids · 2025Article
- Modification of the tumor microenvironment enhances immunity with plasmid gene therapy.Cancer gene therapy · 2024Article
- Transcription factor plasmid binding modulates microtubule interactions and intracellular trafficking during gene transfer.Gene therapy · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Our aim is to characterize the poorly understood mechanisms that influence episomal transgene expression within the nucleus. We found that plasmid DNA micro-injected directly into a nucleus moves into a speckled pattern and occupies less nuclear volume than bovine serum albumin (BSA) or other inert molecules after 4 hours. In addition, plasmids that contain eukaryotic regulatory sequences and actively transcribe transgenes condense into a few select areas of the nucleoplasm and occupy less nuclear volume than bacterial vectors. This suggests that episomal DNA moves in a sequence and transcription-dependent manner. We have also found that plasmids traffic to specific subnuclear domains depending on their sequence. Our experiments show that plasmids with polymerase II regulatory elements will target to nuclear spliceosome regions, while plasmids with the polymerase I promoter often traffic into nucleoli. Further elucidation of intranuclear plasmid trafficking behavior may lead to a better understanding of gene expression, and thereby help to improve basic laboratory techniques and clinical gene therapies.
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Registered trials
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