ArticleAnalytical chemistry2007
Shotgun metabolomics approach for the analysis of negatively charged water-soluble cellular metabolites from mouse heart tissue.
Article in Analytical chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 87 citations in OpenAlex.
- CARDIOKIN1: Computational Assessment of Myocardial Metabolic Capability in Healthy Controls and Patients With Valve Diseases.Circulation · 2021Trial
- Latest Developments in Mass Spectrometry-Based Techniques for Metabolomics Analysis.Advances in experimental medicine and biology · 2026Review
- Integrative proteomic and metabolomic elucidation of cardiomyopathy with in vivo and in vitro models and clinical samples.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Review
- Detecting early myocardial ischemia in rat heart by MALDI imaging mass spectrometry.Scientific reports · 2021Article
- Direct and indirect quantification of phosphate metabolites of nucleoside analogs in biological samples.Journal of pharmaceutical and biomedical analysis · 2020Review
- Metabolic impairments, metal traffic, and dyshomeostasis caused by the antagonistic interaction of cadmium and selenium using organic and inorganic mass spectrometry.Environmental science and pollution research international · 2020Article
- Trends in mass spectrometry imaging for cardiovascular diseases.Analytical and bioanalytical chemistry · 2019Article
- A power law distribution of metabolite abundance levels in mice regardless of the time and spatial scale of analysis.Scientific reports · 2018Article
- Integrative metabolomics as emerging tool to study autophagy regulation.Microbial cell (Graz, Austria) · 2017Review
- Recommendations for quantitative analysis of small molecules by matrix-assisted laser desorption ionization mass spectrometry.Journal of chromatography. A · 2017Review
- Metabolomics in diabetic complications.Molecular bioSystems · 2016Review
- High-energy collision-induced dissociation by MALDI TOF/TOF causes charge-remote fragmentation of steroid sulfates.Journal of the American Society for Mass Spectrometry · 2014Article
- MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals.Metabolites · 2014Article
- MALDI efficiency of metabolites quantitatively associated with their structural properties: a quantitative structure-property relationship (QSPR) approach.Journal of the American Society for Mass Spectrometry · 2014Article
- Mass spectrometry strategies for clinical metabolomics and lipidomics in psychiatry, neurology, and neuro-oncology.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- LC-MS-based Metabolomics of Xenobiotic-induced Toxicities.Computational and structural biotechnology journal · 2013Review
- Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations.Chemistry and physics of lipids · 2012Review
- Matrix-Assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS): A Challenge for Reliable Quantitative Analyses.Mass spectrometry (Tokyo, Japan) · 2012Review
- In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells.Mass spectrometry (Tokyo, Japan) · 2012Review
- Semi-quantitative analyses of metabolic systems of human colon cancer metastatic xenografts in livers of superimmunodeficient NOG mice.Analytical and bioanalytical chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A shotgun metabolomics approach using MALDI-TOF/TOF mass spectrometry was developed for the rapid analysis of negatively charged water-soluble cellular metabolites. Through the use of neutral organic solvents to inactivate endogenous enzyme activities (i.e., methanol/chloroform/H2O extraction), in conjunction with a matrix having minimal background noise (9-amnioacridine), a set of multiplexed conditions was developed that allowed identification of 285 peaks corresponding to negatively charged metabolites from mouse heart extracts. Identification of metabolite peaks was based on mass accuracy and was confirmed by tandem mass spectrometry for 90 of the identified metabolite peaks. Through multiplexing ionization conditions, new suites of metabolites could be ionized and "spectrometric isolation" of closely neighboring peaks for subsequent tandem mass spectrometric interrogation could be achieved. Moreover, assignments of ions from isomeric metabolites and quantitation of their relative abundance was achieved in many cases through tandem mass spectrometry by identification of diagnostic fragmentation ions (e.g., discrimination of ATP from dGTP). The high sensitivity of this approach facilitated the detection of extremely low abundance metabolites including important signaling metabolites such as IP3, cAMP, and cGMP. Collectively, these results identify a multiplexed MALDI-TOF/TOF MS approach for analysis of negatively charged metabolites in mammalian tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.