ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2007
Electron paramagnetic resonance and Mössbauer spectroscopy of intact mitochondria from respiring Saccharomyces cerevisiae.
Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
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- Yeast Mitochondria Import Aqueous FeJournal of the American Chemical Society · 2023Article
- Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei.Metallomics : integrated biometal science · 2022Article
- Yeast cells depleted of the frataxin homolog Yfh1 redistribute cellular iron: Studies using Mössbauer spectroscopy and mathematical modeling.The Journal of biological chemistry · 2022Article
- Mycobacterium tuberculosis requires SufT for Fe-S cluster maturation, metabolism, and survival in vivo.PLoS pathogens · 2022Article
- Direct Detection of the Labile Nickel Pool inJournal of the American Chemical Society · 2021Article
- Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis inThe Journal of biological chemistry · 2020Article
- Treatment of Cells and Tissues with Chromate Maximizes Mitochondrial 2Fe2S EPR Signals.International journal of molecular sciences · 2019Article
- Contribution of Mössbauer spectroscopy to the investigation of Fe/S biogenesis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Recovery of mrs3Δmrs4Δ Saccharomyces cerevisiae Cells under Iron-Sufficient Conditions and the Role of FeBiochemistry · 2018Article
- Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The DUF59 Containing Protein SufT Is Involved in the Maturation of Iron-Sulfur (FeS) Proteins during Conditions of High FeS Cofactor Demand in Staphylococcus aureus.PLoS genetics · 2016Article
- Detection of Labile Low-Molecular-Mass Transition Metal Complexes in Mitochondria.Biochemistry · 2015Article
- Speciation of iron in mouse liver during development, iron deficiency, IRP2 deletion and inflammatory hepatitis.Metallomics : integrated biometal science · 2015Article
- Insights into the iron-ome and manganese-ome of Δmtm1 Saccharomyces cerevisiae mitochondria.Metallomics : integrated biometal science · 2013Article
- Iron content of Saccharomyces cerevisiae cells grown under iron-deficient and iron-overload conditions.Biochemistry · 2013Article
- Article
- Mössbauer and EPR study of iron in vacuoles from fermenting Saccharomyces cerevisiae.Biochemistry · 2011Article
- Friedreich's ataxia variants I154F and W155R diminish frataxin-based activation of the iron-sulfur cluster assembly complex.Biochemistry · 2011Article
- Biophysical investigation of the iron in Aft1-1(up) and Gal-YAH1 Saccharomyces cerevisiae.Biochemistry · 2011Article
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6 authors at 2 institutions in 1 country.
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Abstract
Mitochondria from respiring cells were isolated under anaerobic conditions. Microscopic images were largely devoid of contaminants, and samples consumed O(2) in an NADH-dependent manner. Protein and metal concentrations of packed mitochondria were determined, as was the percentage of external void volume. Samples were similarly packed into electron paramagnetic resonance tubes, either in the as-isolated state or after exposure to various reagents. Analyses revealed two signals originating from species that could be removed by chelation, including rhombic Fe(3+) (g = 4.3) and aqueous Mn(2+) ions (g = 2.00 with Mn-based hyperfine). Three S = 5/2 signals from Fe(3+) hemes were observed, probably arising from cytochrome c peroxidase and the a(3):Cu(b) site of cytochrome c oxidase. Three Fe/S-based signals were observed, with averaged g values of 1.94, 1.90 and 2.01. These probably arise, respectively, from the [Fe(2)S(2)](+) cluster of succinate dehydrogenase, the [Fe(2)S(2)](+) cluster of the Rieske protein of cytochrome bc (1), and the [Fe(3)S(4)](+) cluster of aconitase, homoaconitase or succinate dehydrogenase. Also observed was a low-intensity isotropic g = 2.00 signal arising from organic-based radicals, and a broad signal with g (ave) = 2.02. Mössbauer spectra of intact mitochondria were dominated by signals from Fe(4)S(4) clusters (60-85% of Fe). The major feature in as-isolated samples, and in samples treated with ethylenebis(oxyethylenenitrilo)tetraacetic acid, dithionite or O(2), was a quadrupole doublet with DeltaE (Q) = 1.15 mm/s and delta = 0.45 mm/s, assigned to [Fe(4)S(4)](2+) clusters. Substantial high-spin non-heme Fe(2+) (up to 20%) and Fe(3+) (up to 15%) species were observed. The distribution of Fe was qualitatively similar to that suggested by the mitochondrial proteome.
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