ArticleEuropean biophysics journal : EBJ2007
Organization of reconstituted lipoprotein MexA onto supported lipid membrane.
Article in European biophysics journal : EBJ, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 9 citations in OpenAlex.
- Topical delivery of meropenem via spanlastic carbopol gel:Frontiers in pharmacology · 2025Article
- Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system.Journal of microbiology (Seoul, Korea) · 2019Review
- Assembly and channel opening of outer membrane protein in tripartite drug efflux pumps of Gram-negative bacteria.The Journal of biological chemistry · 2012Article
- Assembly and transport mechanism of tripartite drug efflux systems.Biochimica et biophysica acta · 2009Review
- Quartz crystal microbalance with dissipation monitoring: enabling real-time characterization of biological materials and their interactions.Journal of biomolecular techniques : JBT · 2008Review
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MexA, a periplasmic component of OprM-MexA-MexB tripartite multidrug efflux pump from Pseudomonas aeruginosa, is natively anchored via its fatty acid in the bacteria inner membrane protruding into the periplasm. We used supported lipid bilayer (SLB) to attach the protein to a single leaflet mimicking its perisplamic orientation. For that purpose, we studied the solubilization of DOPC lipid bilayer supported on silica surface with beta-octyl glucoside (betaOG). First we showed that SLBs resist to betaOG concentrations that usually solubilize liposomes. Native form of MexA was directly inserted in the outer leaflet at (betaOG concentrations in a range of 20-25 mM). Second, observations by cryo-electron microscopy (cryoEM) revealed a dense protein layer attached to the surface corresponding to a 13-nm layer of MexA proteins. Analysis of protein densities allows proposing a schematic organization of native MexA inserted in lipid membrane. This structural organization provides further insights with respect to the partially solved structure of the soluble form.
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