ArticleProceedings of the National Academy of Sciences of the United States of America2007
Assays of vacuole fusion resolve the stages of docking, lipid mixing, and content mixing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 84 citations in OpenAlex.
- Exocyst stimulates multiple steps of exocytic SNARE complex assembly and vesicle fusion.Nature structural & molecular biology · 2025Article
- SNARE Proteins in Synaptic Vesicle Fusion.Advances in neurobiology · 2023Article
- Lipids and membrane-associated proteins in autophagy.Protein & cell · 2021Review
- Column-Free Purification Methods for Recombinant Proteins Using Self-Cleaving Aggregating Tags.Polymers · 2018Article
- Sequential actions of phosphatidylinositol phosphates regulate phagosome-lysosome fusion.Molecular biology of the cell · 2018Article
- The NaMolecular biology of the cell · 2018Article
- Molecular mechanisms of atlastin-mediated ER membrane fusion revealed by a FRET-based single-vesicle fusion assay.Scientific reports · 2017Article
- How and why intralumenal membrane fragments form during vacuolar lysosome fusion.Molecular biology of the cell · 2017Article
- Regulation of Exocytotic Fusion Pores by SNARE Protein Transmembrane Domains.Frontiers in molecular neuroscience · 2017Review
- Hemifusion in Synaptic Vesicle Cycle.Frontiers in molecular neuroscience · 2017Review
- The Central Polybasic Region of the Soluble SNARE (Soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor) Vam7 Affects Binding to Phosphatidylinositol 3-Phosphate by the PX (Phox Homology) Domain.The Journal of biological chemistry · 2016Article
- Bioengineered yeast-derived vacuoles with enhanced tissue-penetrating ability for targeted cancer therapy.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- SNAREs support atlastin-mediated homotypic ER fusion in Saccharomyces cerevisiae.The Journal of cell biology · 2015Article
- The Habc domain of the SNARE Vam3 interacts with the HOPS tethering complex to facilitate vacuole fusion.The Journal of biological chemistry · 2015Article
- Vibrio effector protein VopQ inhibits fusion of V-ATPase-containing membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Towards reconstitution of membrane fusion mediated by SNAREs and other synaptic proteins.Critical reviews in biochemistry and molecular biology · 2015Review
- Article
- In vitro assay using engineered yeast vacuoles for neuronal SNARE-mediated membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Plant vacuole morphology and vacuolar trafficking.Frontiers in plant science · 2014Review
- Studying protein-reconstituted proteoliposome fusion with content indicators in vitro.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane fusion entails organelle docking and subsequent mixing of membrane bilayers and luminal compartments. We now present an in vitro assay of fusion, using yeast vacuoles bearing domains of either Fos or Jun fused to complementary halves of beta-lactamase. Upon fusion, these proteins associate to yield beta-lactamase activity. This assay complements the standard fusion assay (activation of pro-Pho8p in protease-deficient vacuoles by proteases from pho8Delta vacuoles). Both the beta-lactamase and pro-Pho8p activation assays of fusion show the same long kinetic delay between SNARE pairing and luminal compartment mixing. Lipid-mixing occurs rapidly after SNARE pairing but well before aqueous compartment mixing. These results support a model in which SNARE pairing leads to rapid hemifusion, followed by slow further lipid rearrangement and aqueous compartment mixing.
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