ArticleMolecular & general genetics : MGG1991
Functional analysis of cis-elements, auxin response and early developmental profiles of the mannopine synthase bidirectional promoter.
Article in Molecular & general genetics : MGG, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 36 citations in OpenAlex.
- Generation of lachrymatory factor synthase-suppressed onion (Frontiers in plant science · 2026Article
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- Benefits of using genomic insulators flanking transgenes to increase expression and avoid positional effects.Scientific reports · 2019Article
- Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.PloS one · 2017Article
- NaturalFrontiers in plant science · 2017Review
- Cosecretion of protease inhibitor stabilizes antibodies produced by plant roots.Plant physiology · 2006Article
- Analysis of the maize polyubiquitin-1 promoter heat shock elements and generation of promoter variants with modified expression characteristics.Transgenic research · 2004Article
- A quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots.Plant cell reports · 2004Article
- A 42 bp fragment of the pmas1' promoter containing an ocs-like element confers a developmental, wound- and chemically inducible expression pattern.Plant molecular biology · 1998Article
- Effect of cadmium treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli.Plant cell reports · 1997Article
- Expression of the isopentenyl transferase gene is regulated by auxin in transgenic tobacco tissues.Transgenic research · 1996Article
- Sequence-specific interactions of wound-inducible nuclear factors with mannopine synthase 2' promoter wound-responsive elements.Plant molecular biology · 1996Article
- Altered Growth and Wood Characteristics in Transgenic Hybrid Aspen Expressing Agrobacterium tumefaciens T-DNA Indoleacetic Acid-Biosynthetic Genes.Plant physiology · 1995Article
- Site-directed mutagenesis of the enhancer region of the 780 gene promoter of T-DNA.Plant molecular biology · 1995Article
- Expression of Agrobacterium rhizogenes auxin biosynthesis genes in transgenic tobacco plants.Plant molecular biology · 1995Article
- Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco.Plant molecular biology · 1994Article
- Analysis of the involvement of ocs-like bZip-binding elements in the differential strength of the bidirectional mas1'2' promoter.Plant physiology · 1994Article
- A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity.Plant molecular biology · 1994Article
- Single-copy T-DNA insertions in Arabidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discs.Plant molecular biology · 1993Article
- Multiple regions of a divergent promoter control the expression of the Agrobacterium rhizogenes aux1 and aux2 plant oncogenes.Molecular & general genetics : MGG · 1993Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dual MAS1'-2' promoter regulating two divergently transcribed mannopine synthase genes has been widely employed in plant expression vectors. As part of an effort towards its rational design as a genetic engineering tool, we have undertaken a functional analysis of the promoter by deletion mutagenesis and by the use of hybrid promoter constructs. Our results indicate that the central region of the intergenic promoter is composed of at least four domains. Three of these contain complementary sequences, which can potentially hybridize to form alternative palindromic structures. These three domains can function cooperatively, and in an orientation-independent manner, in imparting a sevenfold higher expression level at the 2' end relative to the corresponding 1'. The remaining domain is characterized by tracts of repeated A/T-rich elements, and appears to confer the weak activity at the MAS1' promoter end. However, even though this A/T-rich DNA segment is functional, our deletion analysis provided strong evidence that it is completely dispensable for wild-type promoter activity. In addition, the relative distances between these enhancer domains and the 1'-2' TATA-proximal regions can have a pronounced influence on the level of expression in both directions. In young tobacco seedlings, the two promoter ends are expressed in similar, if not identical, tissues in the aerial parts of the plants, but major differences can be observed in roots. Transient expression assays using hybrid promoter constructs showed that cis-elements that can respond to auxin induction signals are redundant in nature, in that they are dispersed throughout the promoter and showed no obvious consensus sequence.
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