ArticleThe Journal of general physiology2007
Responses of rat P2X2 receptors to ultrashort pulses of ATP provide insights into ATP binding and channel gating.
Article in The Journal of general physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Bayesian inference of functional asymmetry in the homotrimeric ligand-gated ion channel P2XCommunications biology · 2025Article
- Subtype-Specific Ligand Binding and Activation Gating in Homomeric and Heteromeric P2X Receptors.Biomolecules · 2024Article
- Fast functional mapping of ligand-gated ion channels.Communications biology · 2023Article
- Enlightening activation gating in P2X receptors.Purinergic signalling · 2022Review
- Unravelling the intricate cooperativity of subunit gating in P2X2 ion channels.Scientific reports · 2020Article
- Modeling a Nociceptive Neuro-Immune Synapse Activated by ATP and 5-HT in Meninges: Novel Clues on Transduction of Chemical Signals Into Persistent or Rhythmic Neuronal Firing.Frontiers in cellular neuroscience · 2020Article
- The Dynamic Behavior of the P2XBiophysical journal · 2016Article
- Article
- Key sites for P2X receptor function and multimerization: overview of mutagenesis studies on a structural basis.Current medicinal chemistry · 2015Review
- Exploring the ATP-binding site of P2X receptors.Frontiers in cellular neuroscience · 2013Review
- An outline of desensitization in pentameric ligand-gated ion channel receptors.Cellular and molecular life sciences : CMLS · 2013Review
- Article
- Activation and regulation of purinergic P2X receptor channels.Pharmacological reviews · 2011Review
- Desensitization of neurotransmitter-gated ion channels during high-frequency stimulation: a comparative study of Cys-loop, AMPA and purinergic receptors.The Journal of physiology · 2011Article
- touché Is required for touch-evoked generator potentials within vertebrate sensory neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- The activation mechanism of alpha1beta2gamma2S and alpha3beta3gamma2S GABAA receptors.The Journal of general physiology · 2010Article
- Loss of myotubularin function results in T-tubule disorganization in zebrafish and human myotubular myopathy.PLoS genetics · 2009Article
- Intermediate closed channel state(s) precede(s) activation in the ATP-gated P2X2 receptor.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
To gain insight into the way that P2X(2) receptors localized at synapses might function, we explored the properties of outside-out patches containing many of these channels as ATP was very rapidly applied and removed. Using a new method to calibrate the speed of exchange of solution over intact patches, we were able to reliably produce applications of ATP lasting <200 micros. For all concentrations of ATP, there was a delay of at least 80 micros between the time when ATP arrived at the receptor and the first detectable flow of inward current. In response to 200-micros pulses of ATP, the time constant of the rising phase of the current was approximately 600 micros. Thus, most channel openings occurred when no free ATP was present. The current deactivated with a time constant of approximately 60 ms. The amplitude of the peak response to a brief pulse of a saturating concentration of ATP was approximately 70% of that obtained during a long application of the same concentration of ATP. Thus, ATP leaves fully liganded channels without producing an opening at least 30% of the time. Extensive kinetic modeling revealed three different schemes that fit the data well, a sequential model and two allosteric models. To account for the delay in opening at saturating ATP, it was necessary to incorporate an intermediate closed state into all three schemes. These kinetic properties indicate that responses to ATP at synapses that use homomeric P2X(2) receptors would be expected to greatly outlast the duration of the synaptic ATP transient produced by a single presynaptic spike. Like NMDA receptors, P2X(2) receptors provide the potential for complex patterns of synaptic integration over a time scale of hundreds of milliseconds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.