ArticleThe Journal of general physiology2007
Modulation of extracellular proton fluxes from retinal horizontal cells of the catfish by depolarization and glutamate.
Article in The Journal of general physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Non-invasive micro-test technology and applications.Biophysics reports · 2025Article
- pH in the vertebrate retina and its naturally occurring and pathological changes.Progress in retinal and eye research · 2025Review
- ATP-mediated increase in HJournal of neurophysiology · 2024Article
- Extracellular ATP-Induced Alterations in Extracellular HFrontiers in cellular neuroscience · 2021Article
- Review and Hypothesis: A Potential Common Link Between Glial Cells, Calcium Changes, Modulation of Synaptic Transmission, Spreading Depression, Migraine, and Epilepsy-HFrontiers in cellular neuroscience · 2021Article
- Spontaneous action potentials in retinal horizontal cells of goldfish (Journal of neurophysiology · 2019Article
- Localizing Proton-Mediated Inhibitory Feedback at the Retinal Horizontal Cell-Cone Synapse with Genetically-Encoded pH Probes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Activation of retinal glial (Müller) cells by extracellular ATP induces pronounced increases in extracellular H+ flux.PloS one · 2018Article
- How do horizontal cells 'talk' to cone photoreceptors? Different levels of complexity at the cone-horizontal cell synapse.The Journal of physiology · 2017Review
- Membrane-associated guanylate kinase scaffolds organize a horizontal cell synaptic complex restricted to invaginating contacts with photoreceptors.The Journal of comparative neurology · 2017Article
- Calcium dynamics and regulation in horizontal cells of the vertebrate retina: lessons from teleosts.Journal of neurophysiology · 2017Review
- Fluorescent Visualization of Cellular Proton Fluxes.Cell chemical biology · 2016Article
- The function and regulation of acid-sensing ion channels (ASICs) and the epithelial Na(+) channel (ENaC): IUPHAR Review 19.British journal of pharmacology · 2016Review
- Lateral Inhibition in the Vertebrate Retina: The Case of the Missing Neurotransmitter.PLoS biology · 2015Article
- Autocrine boost of NMDAR current in hippocampal CA1 pyramidal neurons by a PMCA-dependent, perisynaptic, extracellular pH shift.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Article
- Fluorescent imaging reports an extracellular alkalinization induced by glutamatergic activation of isolated retinal horizontal cells.Journal of neurophysiology · 2014Article
- Calcium channels in rat horizontal cells regulate feedback inhibition of photoreceptors through an unconventional GABA- and pH-sensitive mechanism.The Journal of physiology · 2013Article
- Engaging Undergraduates in a Unique Neuroscience Research Opportunity: A Collaborative Research Experience Between a Primarily Undergraduate Institution (PUI) and a Major Research Institution.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2013Article
- Distinctive patterns of alterations in proton efflux from goldfish retinal horizontal cells monitored with self-referencing H⁺-selective electrodes.The European journal of neuroscience · 2012Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Self-referencing H(+)-selective microelectrodes were used to measure extracellular proton fluxes from cone-driven horizontal cells isolated from the retina of the catfish (Ictalurus punctatus). The neurotransmitter glutamate induced an alkalinization of the area adjacent to the external face of the cell membrane. The effect of glutamate occurred regardless of whether the external solution was buffered with 1 mM HEPES, 3 mM phosphate, or 24 mM bicarbonate. The AMPA/kainate receptor agonist kainate and the NMDA receptor agonist N-methyl-D-aspartate both mimicked the effect of glutamate. The effect of kainate on proton flux was inhibited by the AMPA/kainate receptor blocker CNQX, and the effect of NMDA was abolished by the NMDA receptor antagonist DAP-5. Metabotropic glutamate receptor agonists produced no alteration in proton fluxes from horizontal cells. Depolarization of cells either by increasing extracellular potassium or directly by voltage clamp also produced an alkalinization adjacent to the cell membrane. The effects of depolarization on proton flux were blocked by 10 microM nifedipine, an inhibitor of L-type calcium channels. The plasmalemma Ca(2+/)H(+) ATPase (PMCA) blocker 5(6)-carboxyeosin also significantly reduced proton flux modulation by glutamate. Our results are consistent with the hypothesis that glutamate-induced extracellular alkalinizations arise from activation of the PMCA pump following increased intracellular calcium entry into cells. This process might help to relieve suppression of photoreceptor neurotransmitter release that results from exocytosed protons from photoreceptor synaptic terminals. Our findings argue strongly against the hypothesis that protons released by horizontal cells act as the inhibitory feedback neurotransmitter that creates the surround portion of the receptive fields of retinal neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.