ArticleMolecular and cellular biology2007
Undamaged DNA transmits and enhances DNA damage checkpoint signals in early embryos.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Stage-Specific Effects of Ionizing Radiation during Early Development.International journal of molecular sciences · 2020Review
- Timing the Drosophila Mid-Blastula Transition: A Cell Cycle-Centered View.Trends in genetics : TIG · 2016Review
- Regulation of polo-like kinase 1 by DNA damage and PP2A/B55α.Cell cycle (Georgetown, Tex.) · 2015Article
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- Article
- Greatwall and Polo-like kinase 1 coordinate to promote checkpoint recovery.The Journal of biological chemistry · 2011Article
- DNA damage during G2 phase does not affect cell cycle progression of the green alga Scenedesmus quadricauda.PloS one · 2011Article
- Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes.Molecular reproduction and development · 2011Article
- Intracellular traffic of oligodeoxynucleotides in and out of the nucleus: effect of exportins and DNA structure.Oligonucleotides · 2010Article
- A novel role for greatwall kinase in recovery from DNA damage.Cell cycle (Georgetown, Tex.) · 2010Article
- Repo-man controls a protein phosphatase 1-dependent threshold for DNA damage checkpoint activation.Current biology : CB · 2010Article
- Discovery of a distinct domain in cyclin A sufficient for centrosomal localization independently of Cdk binding.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Smicl is required for phosphorylation of RNA polymerase II and affects 3'-end processing of RNA at the midblastula transition in Xenopus.Development (Cambridge, England) · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Xenopus laevis embryos, the midblastula transition (MBT) at the 12th cell division marks initiation of critical developmental events, including zygotic transcription and the abrupt inclusion of gap phases into the cell cycle. Interestingly, although an ionizing radiation-induced checkpoint response is absent in pre-MBT embryos, introduction of a threshold amount of undamaged plasmid or sperm DNA allows a DNA damage checkpoint response to be activated. We show here that undamaged threshold DNA directly participates in checkpoint signaling, as judged by several dynamic changes, including H2AX phosphorylation, ATM phosphorylation and loading onto chromatin, and Chk1/Chk2 phosphorylation and release from nuclear DNA. These responses on physically separate threshold DNA require gamma-H2AX and are triggered by an ATM-dependent soluble signal initiated by damaged DNA. The signal persists in egg extracts even after damaged DNA is removed from the system, indicating that the absence of damaged DNA is not sufficient to end the checkpoint response. The results identify a novel mechanism by which undamaged DNA enhances checkpoint signaling and provide an example of how the transition to cell cycle checkpoint activation during development is accomplished by maternally programmed increases in the DNA-to-cytoplasm ratio.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.