ArticleTraffic (Copenhagen, Denmark)2007
Efficient electroporation of DNA and protein into confluent and differentiated epithelial cells in culture.
Article in Traffic (Copenhagen, Denmark), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- External stimuli-responsive drug delivery to the posterior segment of the eye.Drug delivery · 2025Review
- Cell cycle-regulated tug-of-war between microtubule motors positions major trafficking organelles.bioRxiv : the preprint server for biology · 2025Article
- Establishment of a non-integrated induced pluripotent stem cell line derived from human chorionic villi cells.Journal of clinical laboratory analysis · 2022Article
- Article
- Article
- Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation.The Journal of veterinary medical science · 2020Article
- Detection of Microtubule Nucleation Hotspots at the Golgi.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Regulation of Glucose-Dependent Golgi-Derived Microtubules by cAMP/EPAC2 Promotes Secretory Vesicle Biogenesis in Pancreatic β Cells.Current biology : CB · 2019Article
- A platform for assessing outer segment fate in primary human fetal RPE cultures.Experimental eye research · 2019Article
- Nonrandom γ-TuNA-dependent spatial pattern of microtubule nucleation at the Golgi.Molecular biology of the cell · 2017Article
- Internalized TSH receptors en route to the TGN induce local GNature communications · 2017Article
- Galectin-4-mediated transcytosis of transferrin receptor.Journal of cell science · 2014Article
- Influenza entry pathways in polarized MDCK cells.Biochemical and biophysical research communications · 2014Article
- The kinesin KIF16B mediates apical transcytosis of transferrin receptor in AP-1B-deficient epithelia.The EMBO journal · 2013Article
- Design and implementation of a microelectrode assembly for use on noncontact in situ electroporation of adherent cells.The Journal of membrane biology · 2012Article
- A FRET sensor for non-invasive imaging of amyloid formation in vivo.Chemphyschem : a European journal of chemical physics and physical chemistry · 2011Article
- Clathrin adaptor AP1B controls adenovirus infectivity of epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- New mechanisms for non-porative ultrasound stimulation of cargo delivery to cell cytosol with targeted perfluorocarbon nanoparticles.Nanotechnology · 2008Article
- Clathrin is a key regulator of basolateral polarity.Nature · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Electroporation-mediated delivery of molecules is a procedure widely used for transfecting complementary DNA in bacteria, mammalian and plant cells. This technique has proven very efficient for the introduction of macromolecules into cells in suspension culture and even into cells in their native tissue environment, e.g. retina and embryonic tissues. However, in spite of several attempts to date, there are no well-established procedures to electroporate polarized epithelial cells adhering to a tissue culture substrate (glass, plastic or filter). We report here the development of a simple procedure that uses available commercial equipment and works efficiently and reproducibly for a variety of epithelial cell lines in culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.