ArticleGenetics2007
A SAGA-independent function of SPT3 mediates transcriptional deregulation in a mutant of the Ccr4-not complex in Saccharomyces cerevisiae.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability.Cells · 2023Article
- Review
- Improvement of Lead Tolerance of Saccharomyces cerevisiae by Random Mutagenesis of Transcription Regulator SPT3.Applied biochemistry and biotechnology · 2018Article
- Def1 interacts with TFIIH and modulates RNA polymerase II transcription.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA.Nucleic acids research · 2017Article
- The Ccr4-Not complex is a key regulator of eukaryotic gene expression.Wiley interdisciplinary reviews. RNA · 2016Review
- Improvement of experimental testing and network training conditions with genome-wide microarrays for more accurate predictions of drug gene targets.BMC systems biology · 2014Article
- Spatial localization of co-regulated genes exceeds genomic gene clustering in the Saccharomyces cerevisiae genome.Nucleic acids research · 2013Article
- The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control.ISRN molecular biology · 2013Review
- Histone acetylation, acetyltransferases, and ataxia--alteration of histone acetylation and chromatin dynamics is implicated in the pathogenesis of polyglutamine-expansion disorders.Advances in protein chemistry and structural biology · 2010Review
- The CCR4-NOT complex physically and functionally interacts with TRAMP and the nuclear exosome.PloS one · 2009Article
- Specific roles for the Ccr4-Not complex subunits in expression of the genome.RNA (New York, N.Y.) · 2009Article
- Article
- Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conserved multi-subunit Ccr4-Not complex regulates gene expression in diverse ways. In this work, we characterize the suppression of temperature sensitivity associated with a mutation in the gene encoding the scaffold subunit of the Ccr4-Not complex, NOT1, by the deletion of SPT3. We determine that the deletion of SPT3, but not the deletion of genes encoding other subunits of the SAGA complex, globally suppresses transcriptional defects of not1-2. We find that transcriptional activation in not1-2 is associated with increased binding of TFIID and SAGA at promoters of upregulated genes, and this is suppressed by the deletion of SPT3. Interestingly, Spt3p-dependent activation of transcription occurs in not1-2 even if the SAGA complex is disrupted by the deletion of SPT7 that encodes a subunit of SAGA required for its integrity. Consistent with a SAGA-independent function of Spt3p, the deletion of SPT3 displays synthetic phenotypes when combined with a deletion of SPT7. Taken together, our results provide a new view of the Spt3 protein by identifying a SAGA-independent function of this protein that is functionally linked to the Ccr4-Not complex.
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