ArticleEukaryotic cell2007
FEA1, FEA2, and FRE1, encoding two homologous secreted proteins and a candidate ferrireductase, are expressed coordinately with FOX1 and FTR1 in iron-deficient Chlamydomonas reinhardtii.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 137 citations in OpenAlex.
- Client distribution between Chlamydomonas FDX1 and FDX2 in carbon, nitrogen and sulfur assimilation.bioRxiv : the preprint server for biology · 2026Article
- Targeted genetic manipulation and yeast-like evolutionary genomics in the green alga Auxenochlorella.The Plant cell · 2025Article
- Targeted genetic manipulation and yeast-like evolutionary genomics in the green algabioRxiv : the preprint server for biology · 2025Article
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- Efficient secretory production of recombinant proteins in microalgae using an exogenous signal peptide.Frontiers in microbiology · 2025Article
- Cell differentiation controls iron assimilation in a choanoflagellate.bioRxiv : the preprint server for biology · 2024Article
- Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium.Photosynthesis research · 2024Article
- Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis.Nature communications · 2024Article
- Distinct function of Chlamydomonas CTRA-CTR transporters in Cu assimilation and intracellular mobilization.Metallomics : integrated biometal science · 2024Article
- Conserved cobalamin acquisition protein 1 is essential for vitamin B12 uptake in both Chlamydomonas and Phaeodactylum.Plant physiology · 2024Article
- Optimizing Fe Nutrition for Algal Growth.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Iron-dependent mutualism betweenISME communications · 2022Article
- Long-term acclimation to cadmium exposure reveals extensive phenotypic plasticity in Chlamydomonas.Plant physiology · 2021Article
- A quick journey into the diversity of iron uptake strategies in photosynthetic organisms.Plant signaling & behavior · 2021Review
- Single-cell RNA sequencing of batch Chlamydomonas cultures reveals heterogeneity in their diurnal cycle phase.The Plant cell · 2021Article
- Crystallographic analysis and phasing of iron-assimilating protein 1 (FEA1) from Chlamydomonas reinhardtii.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Single-cell visualization and quantification of trace metals inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Iron metabolism strategies in diatoms.Journal of experimental botany · 2021Review
- Comparative secretome analysis between salinity-tolerant and control Chlamydomonas reinhardtii strains.Planta · 2021Article
- Fe-S Protein Synthesis in Green Algae Mitochondria.Plants (Basel, Switzerland) · 2021Review
3 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
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Abstract
Previously, we had identified FOX1 and FTR1 as iron deficiency-inducible components of a high-affinity copper-dependent iron uptake pathway in Chlamydomonas. In this work, we survey the version 3.0 draft genome to identify a ferrireductase, FRE1, and two ZIP family proteins, IRT1 and IRT2, as candidate ferrous transporters based on their increased expression in iron-deficient versus iron-replete cells. In a parallel proteomic approach, we identified FEA1 and FEA2 as the major proteins secreted by iron-deficient Chlamydomonas reinhardtii. The recovery of FEA1 and FEA2 from the medium of Chlamydomonas strain CC425 cultures is strictly correlated with iron nutrition status, and the accumulation of the corresponding mRNAs parallels that of the Chlamydomonas FOX1 and FTR1 mRNAs, although the magnitude of regulation is more dramatic for the FEA genes. Like the FOX1 and FTR1 genes, the FEA genes do not respond to copper, zinc, or manganese deficiency. The 5' flanking untranscribed sequences from the FEA1, FTR1, and FOX1 genes confer iron deficiency-dependent expression of ARS2, suggesting that the iron assimilation pathway is under transcriptional control by iron nutrition. Genetic analysis suggests that the secreted proteins FEA1 and FEA2 facilitate high-affinity iron uptake, perhaps by concentrating iron in the vicinity of the cell. Homologues of FEA1 and FRE1 were identified previously as high-CO(2)-responsive genes, HCR1 and HCR2, in Chlorococcum littorale, suggesting that components of the iron assimilation pathway are responsive to carbon nutrition. These iron response components are placed in a proposed iron assimilation pathway for Chlamydomonas.
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