ArticleApplied and environmental microbiology2007
Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptome analysis and its application to whole-cell heavy-metal detection systems.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 55 citations in OpenAlex.
- Yeast Biosensors for the Safety of Fermented Beverages.Biosensors · 2026Review
- Transcription regulation strategies in methylotrophs: progress and challenges.Bioresources and bioprocessing · 2022Review
- The Use of Yeast in Biosensing.Microorganisms · 2022Review
- Expanding the promoter toolbox for metabolic engineering of methylotrophic yeasts.Applied microbiology and biotechnology · 2022Review
- Yeast-Based Biosensors: Current Applications and New Developments.Biosensors · 2020Review
- Discovery and identification of medium-chain fatty acid responsive promoters inEngineering in life sciences · 2020Article
- Yeast Expression Systems: Overview and Recent Advances.Molecular biotechnology · 2019Review
- In vivo biosensors: mechanisms, development, and applications.Journal of industrial microbiology & biotechnology · 2018Article
- Enhanced Expression of Pullulanase inFrontiers in microbiology · 2018Article
- Heavy metal whole-cell biosensors using eukaryotic microorganisms: an updated critical review.Frontiers in microbiology · 2015Article
- Global transcriptome analysis of hexavalent chromium stress responses in Staphylococcus aureus LZ-01.Ecotoxicology (London, England) · 2014Article
- Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica.Journal of microbiology (Seoul, Korea) · 2014Article
- Article
- Functional analysis of a Hansenula polymorpha MNN2-2 homologue encoding a putative UDP-N-acetylglucosamine transporter localized in the endoplasmic reticulum.Journal of microbiology (Seoul, Korea) · 2011Article
- Whole-cell biosensors for detection of heavy metal ions in environmental samples based on metallothionein promoters from Tetrahymena thermophila.Microbial biotechnology · 2011Article
- Development of a conditional gene expression system using a zearalenone-inducible promoter for the ascomycete fungus Gibberella zeae.Applied and environmental microbiology · 2010Article
- Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis.BMC genomics · 2010Article
- Process development in Hansenula polymorpha and Arxula adeninivorans, a re-assessment.Microbial cell factories · 2009Article
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Authors and funding
9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The genomewide gene expression profiling of the methylotrophic yeast Hansenula polymorpha exposed to cadmium (Cd) allowed us to identify novel genes responsive to Cd treatment. To select genes whose promoters can be useful for construction of a cellular Cd biosensor, we further analyzed a set of H. polymorpha genes that exhibited >6-fold induction upon treatment with 300 muM Cd for 2 h. The putative promoters, about 1,000-bp upstream fragments, of these genes were fused with the yeast-enhanced green fluorescence protein (GFP) gene. The resultant reporter cassettes were introduced into H. polymorpha to evaluate promoter strength and specificity. The promoter derived from the H. polymorpha SEO1 gene (HpSEO1) was shown to drive most strongly the expression of GFP upon Cd treatment among the tested promoters. The Cd-inducible activity was retained in the 500-bp deletion fragment of the HpSEO1 promoter but was abolished in the further truncated 250-bp fragment. The 500-bp HpSEO1 promoter directed specific expression of GFP upon exposure to Cd in a dose-dependent manner, with Cd detection ranging from 1 to 900 muM. Comparative analysis of the Saccharomyces cerevisiae SEO1 (ScSEO1) promoter revealed that the ScSEO1 promoter has a broader specificity for heavy metals and is responsive to arsenic and mercury in addition to Cd. Our data demonstrate the potential use of the HpSEO1 promoter as a bioelement in whole-cell biosensors to monitor heavy metal contamination, particularly Cd.
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