ArticleApplied and environmental microbiology2007
Characteristics of Gloeophyllum trabeum alcohol oxidase, an extracellular source of H2O2 in brown rot decay of wood.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 120 citations in OpenAlex.
- Genuine Directed Evolution In Test Tube (GENie).bioRxiv : the preprint server for biology · 2026Article
- Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases.Synthetic and systems biotechnology · 2025Article
- Novel Basidiomycetous Alcohol Oxidase fromInternational journal of molecular sciences · 2024Article
- Biosynthetic Strategies of Berberine Bridge Enzyme-like Flavoprotein Oxidases toward Structural Diversification in Natural Product Biosynthesis.Biochemistry · 2024Review
- A Combination of Transcriptome and Enzyme Activity Analysis Unveils Key Genes and Patterns of Corncob Lignocellulose Degradation byJournal of fungi (Basel, Switzerland) · 2024Article
- First Description of Non-Enzymatic Radical-Generating Mechanisms Adopted byJournal of fungi (Basel, Switzerland) · 2023Article
- Properties, Physiological Functions and Involvement of Basidiomycetous Alcohol Oxidase in Wood Degradation.International journal of molecular sciences · 2022Review
- Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors.Nature communications · 2022Article
- Basidiomycota Fungi and ROS: Genomic Perspective on Key Enzymes Involved in Generation and Mitigation of Reactive Oxygen Species.Frontiers in fungal biology · 2022Review
- Retracted and Republished from: "Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot FungusApplied and environmental microbiology · 2021Article
- A Multiomic Approach to Understand HowJournal of fungi (Basel, Switzerland) · 2021Article
- Pecularities and applications of aryl-alcohol oxidases from fungi.Applied microbiology and biotechnology · 2021Review
- Transcriptome analysis of the brown rot fungus Gloeophyllum trabeum during lignocellulose degradation.PloS one · 2020Article
- Transcriptome analysis of differential gene expression in Dichomitus squalens during interspecific mycelial interactions and the potential link with laccase induction.Journal of microbiology (Seoul, Korea) · 2019Article
- The GMC superfamily of oxidoreductases revisited: analysis and evolution of fungal GMC oxidoreductases.Biotechnology for biofuels · 2019Article
- Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.Microbiology and molecular biology reviews : MMBR · 2018Review
- Oxidative Damage Control during Decay of Wood by Brown Rot Fungus Using Oxygen Radicals.Applied and environmental microbiology · 2018Article
- Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.Biochemistry · 2018Article
- Multi-omic Analyses of Extensively Decayed Pinus contorta Reveal Expression of a Diverse Array of Lignocellulose-Degrading Enzymes.Applied and environmental microbiology · 2018Article
- Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola.Applied and environmental microbiology · 2018Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel alcohol oxidase (AOX) has been purified from mycelial pellets of the wood-degrading basidiomycete Gloeophyllum trabeum and characterized as a homooctameric nonglycosylated protein with native and subunit molecular masses of 628 and 72.4 kDa, containing noncovalently bonded flavin adenine dinucleotide. The isolated AOX cDNA contained an open reading frame of 1,953 bp translating into a polypeptide of 651 amino acids displaying 51 to 53% identity with other published fungal AOX amino acid sequences. The enzyme catalyzed the oxidation of short-chain primary aliphatic alcohols with a preference for methanol (K(m) = 2.3 mM, k(cat) = 15.6 s(-1)). Using polyclonal antibodies and immunofluorescence staining, AOX was localized on liquid culture hyphae and extracellular slime in sections from degraded wood and on cotton fibers. Transmission electron microscopy immunogold labeling localized the enzyme in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there was no labeling of hyphal peroxisomes. AOX was further shown to be associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers. The differences in AOX targeting compared to the known yeast peroxisomal localization were traced to a unique C-terminal sequence of the G. trabeum oxidase, which is apparently responsible for the protein's different translocation. The extracellular distribution and the enzyme's abundance and preference for methanol, potentially available from the demethylation of lignin, all point to a possible role for AOX as a major source of H(2)O(2), a component of Fenton's reagent implicated in the generally accepted mechanisms for brown rot through the production of highly destructive hydroxyl radicals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.