ArticleJournal of bacteriology2007
Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 85 citations in OpenAlex.
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- A host-pathogen metabolic synchrony that facilitates disease tolerance.Nature communications · 2025Article
- Bacterial osmoprotectants-a way to survive in saline conditions and potential crop allies.FEMS microbiology reviews · 2025Review
- Bacterial cell volume regulation and the importance of cyclic di-AMP.Microbiology and molecular biology reviews : MMBR · 2024Review
- An atypical 3-ketoacyl ACP synthase III required for acyl homoserine lactone synthesis inApplied and environmental microbiology · 2024Article
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- Light cues induce protective anticipation of environmental water loss in terrestrial bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genomic diversity and biosynthetic capabilities of sponge-associated chlamydiae.The ISME journal · 2022Article
- Salinity of irrigation water selects distinct bacterial communities associated with date palm (Phoenix dactylifera L.) root.Scientific reports · 2022Article
- Proline confers acid stress tolerance to Bacillus megaterium G18.Scientific reports · 2022Article
- In planta transcriptomics reveals conflicts between pattern-triggered immunity and the AlgU sigma factor regulon.PloS one · 2022Article
- Cell Envelope Stress Response in Pseudomonas aeruginosa.Advances in experimental medicine and biology · 2022Article
- Stressed out: Bacterial response to high salinity using compatible solute biosynthesis and uptake systems, lessons fromComputational and structural biotechnology journal · 2021Review
- A decade of research on the second messenger c-di-AMP.FEMS microbiology reviews · 2020Review
- Investigations of Dimethylglycine, Glycine Betaine, and Ectoine Uptake by a Betaine-Carnitine-Choline Transporter Family Transporter with Diverse Substrate Specificity inJournal of bacteriology · 2020Article
- CosR Is a Global Regulator of the Osmotic Stress Response with Widespread Distribution among Bacteria.Applied and environmental microbiology · 2020Article
- Choline-betaine pathway contributes to hyperosmotic stress and subsequent lethal stress resistance inJournal of biosciences · 2020Article
- The Impact of Carnitine on Dietary Fiber and Gut Bacteria Metabolism and Their Mutual Interaction in Monogastrics.International journal of molecular sciences · 2018Review
- Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria.Journal of bacteriology · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant pathogen Pseudomonas syringae may cope with osmotic stress on plants, in part, by importing osmoprotective compounds. In this study, we found that P. syringae pv. tomato strain DC3000 was distinct from most bacterial species in deriving greater osmoprotection from exogenous choline than from glycine betaine. This superior osmoprotection was correlated with a higher capacity for uptake of choline than for uptake of glycine betaine. Of four putative osmoregulatory ABC transporters in DC3000, one, designated OpuC, functioned as the primary or sole transporter for glycine betaine and as one of multiple transporters for choline under high osmolarity. Surprisingly, the homolog of the well-characterized ProU transporter from Escherichia coli and Salmonella enterica serovar Typhimurium did not function in osmoprotection. The P. syringae pv. tomato OpuC transporter was more closely related to the Bacillus subtilis and Listeria monocytogenes OpuC transporters than to known osmoprotectant transporters in gram-negative bacteria based on sequence similarity and genetic arrangement. The P. syringae pv. tomato OpuC transporter had a high affinity for glycine betaine, a low affinity for choline, and a broad substrate specificity that included acetylcholine, carnitine, and proline betaine. Tandem cystathionine-beta-synthase (CBS) domains in the ATP-binding component of OpuC were required for transporter function. The presence of these CBS domains was correlated with osmoregulatory function among the putative transporters examined in DC3000 and was found to be predictive of functional osmoregulatory transporters in other pseudomonads. These results provide the first functional evaluation of an osmoprotectant transporter in a Pseudomonas species and demonstrate the usefulness of the CBS domains as predictors of osmoregulatory activity.
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