ArticleProtein science : a publication of the Protein Society2007
Production of selenomethionyl-derivatized proteins in baculovirus-infected insect cells.
Article in Protein science : a publication of the Protein Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Equi-MOI ratio for rapid baculovirus-mediated multiprotein co-expression in insect cells integrating selenomethionine for structural studies.FEBS open bio · 2025Article
- The effect of pH on the structure of Bluetongue virus VP5.The Journal of general virology · 2024Article
- Crystal structure of human PLD1 provides insight into activation by PI(4,5)PNature chemical biology · 2020Article
- NELL2-Robo3 complex structure reveals mechanisms of receptor activation for axon guidance.Nature communications · 2020Article
- A practical method for efficient and optimal production of Seleno-methionine-labeled recombinant protein complexes in the insect cells.Protein science : a publication of the Protein Society · 2019Article
- Rif1 maintains telomeres and mediates DNA repair by encasing DNA ends.Nature structural & molecular biology · 2017Article
- A molecular engineering toolbox for the structural biologist.Quarterly reviews of biophysics · 2017Review
- An overview of heavy-atom derivatization of protein crystals.Acta crystallographica. Section D, Structural biology · 2016Review
- Atomic-Resolution Structures of the APC/C Subunits Apc4 and the Apc5 N-Terminal Domain.Journal of molecular biology · 2015Article
- Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome.Journal of molecular biology · 2015Article
- Crystal structure of the human COP9 signalosome.Nature · 2014Article
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- Mutation of high-affinity methionine permease contributes to selenomethionyl protein production in Saccharomyces cerevisiae.Applied and environmental microbiology · 2010Article
- Selenium incorporation using recombinant techniques.Acta crystallographica. Section D, Biological crystallography · 2010Review
- Structure of a protein phosphatase 2A holoenzyme: insights into B55-mediated Tau dephosphorylation.Molecular cell · 2008Article
- Strategies for manufacturing recombinant adeno-associated virus vectors for gene therapy applications exploiting baculovirus technology.Briefings in functional genomics & proteomics · 2008Review
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3 authors.
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Abstract
A protocol is described for the production of both intracellularly expressed and secreted selenomethionyl-derivatized recombinant proteins in baculovirus-infected insect cells. The method results in the production of recombinant soluble proteins with an SeMet occupancy of approximately 75% and with a recovery of approximately 20% that of native protein expression. The method is independent of the percentage methionine content of the protein and is reliable and consistent. Similar results are obtained using either Spodoptera frugiperda Sf9 or Trichoplusia ni High Five insect cells as the expression host, and when cultures are grown in either shake flasks or in Wave BioReactors.
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