ArticleThe Journal of physiology2007
Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium.
Article in The Journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 72 citations in OpenAlex.
- Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.Chemical senses · 2023Article
- Intricacies of GABAInternational journal of molecular sciences · 2021Review
- The cyclic AMP signaling pathway in the rodent main olfactory system.Cell and tissue research · 2021Review
- Biochemical properties of the sensitivity to GABAFish physiology and biochemistry · 2018Article
- Tracking of unfamiliar odors is facilitated by signal amplification through anoctamin 2 chloride channels in mouse olfactory receptor neurons.Physiological reports · 2017Article
- Chloride Dysregulation, Seizures, and Cerebral Edema: A Relationship with Therapeutic Potential.Trends in neurosciences · 2017Review
- Cyclic-nucleotide-gated cation current and Ca2+-activated Cl current elicited by odorant in vertebrate olfactory receptor neurons.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Properties of an optogenetic model for olfactory stimulation.The Journal of physiology · 2016Article
- Differential expression of pancreatic protein and chemosensing receptor mRNAs in NKCC1-null intestine.World journal of gastrointestinal pathophysiology · 2016Article
- Plasma Membrane Targeting of Endogenous NKCC2 in COS7 Cells Bypasses Functional Golgi Cisternae and Complex N-Glycosylation.Frontiers in cell and developmental biology · 2016Article
- Ion transporter NKCC1, modulator of neurogenesis in murine olfactory neurons.The Journal of biological chemistry · 2015Article
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- Chloride Accumulators NKCC1 and AE2 in Mouse GnRH Neurons: Implications for GABAA Mediated Excitation.PloS one · 2015Article
- Standardized, systemic phenotypic analysis of Slc12a1I299F mutant mice.Journal of biomedical science · 2014Article
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- Article
- Genomics of mature and immature olfactory sensory neurons.The Journal of comparative neurology · 2012Article
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- Ca2+-activated Cl− currents are dispensable for olfaction.Nature neuroscience · 2011Article
- Mechanisms of chloride uptake in frog olfactory receptor neurons.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
When olfactory receptor neurons respond to odours, a depolarizing Cl(-) efflux is a substantial part of the response. This requires that the resting neuron accumulate Cl(-) against an electrochemical gradient. In isolated olfactory receptor neurons, the Na(+)-K(+)-2Cl(-) cotransporter NKCC1 is essential for Cl(-) accumulation. However, in intact epithelium, a robust electrical olfactory response persists in mice lacking NKCC1. This response is largely due to a neuronal Cl(-) efflux. It thus appears that NKCC1 is an important part of a more complex system of Cl(-) accumulation. To identify the remaining transport proteins, we first screened by RT-PCR for 21 Cl(-) transporters in mouse nasal tissue containing olfactory mucosa. For most of the Cl(-) transporters, the presence of mRNA was demonstrated. We also investigated the effects of pharmacological block or genetic ablation of Cl(-) transporters on the olfactory field potential, the electroolfactogram (EOG). Mice lacking the common Cl(-)/HCO(3)(-) exchanger AE2 had normal EOGs. Block of NKCC cotransport with bumetanide reduced the EOG in epithelia from wild-type mice but had no effect in mice lacking NKCC1. Hydrochlorothiazide, a blocker of the Na(+)-Cl(-) cotransporter, had only a small effect. DIDS, a blocker of some KCC cotransporters and Cl(-)/HCO(3)(-) exchangers, reduced the EOG in epithelia from both wild-type and NKCC1 knockout mice. A combination of bumetanide and DIDS decreased the response more than either drug alone. However, no combination of drugs completely abolished the Cl(-) component of the response. These results support the involvement of both NKCC1 and one or more DIDS-sensitive transporters in Cl(-) accumulation in olfactory receptor neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.