ArticleMolecular biotechnology2007
Detection and typing of BKV, JCV, and SV40 by multiplex nested polymerase chain reaction.
Article in Molecular biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 4 citations in OpenAlex.
- JC virus PCR assay reporting for PML: promises, perils, and pitfalls.Journal of neurology · 2025Review
- Nested PCR detection of JC polyomavirus large T-antigen in prostate cancer tissues: a case-control analysis in a Sudanese population.Journal of the Egyptian National Cancer Institute · 2025Article
- A globally applicable PCR-based detection and discrimination of BK and JC polyomaviruses.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
- Real time PCR TaqMan assays for detection of polyomaviruses KIV and WUV in clinical samples.Journal of virological methods · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex nested polymerase chain reaction (PCR) method was developed for detecting and differentiating simultaneously the DNA of polyomaviruses JC, BK, and SV40 in a single tube. In the first amplification step the same set of primers was used to amplify a conserved DNA region of the large T antigen gene of JCV, BKV, and SV40. The second round was carried out using a set of primers designed to obtain products of different size for each related virus. Subsequently, the sensitivity of the multiplex nested PCR was maximized by optimizing parameters such as primer, magnesium, and dNTP concentrations. The sensitivity of the method ranged between 1 and 10 copies of the polyomavirus genome. The assay was then used for detecting polyomavirus DNA in urine, serum, and biopsy specimens from renal transplant recipients. Based on the results obtained, the multiplex nested PCR developed in our study represents a useful tool for supporting the diagnosis of polyomavirus infection and could be used for epidemiological purposes and to better define the role of polyomaviruses in human pathology.
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Registered trials
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