ArticleJournal of clinical microbiology2007
DNA pyrosequencing-based bacterial pathogen identification in a pediatric hospital setting.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 71 citations in OpenAlex.
- Short SignatureMicrobiology spectrum · 2022Article
- Recent Advances in the Use of Molecular Methods for the Diagnosis of Bacterial Infections.Pathogens (Basel, Switzerland) · 2022Review
- Rothia mucilaginosa Infections in Pediatric Cancer Patients.Journal of the Pediatric Infectious Diseases Society · 2021Article
- High-throughput identification and diagnostics of pathogens and pests: Overview and practical recommendations.Molecular ecology resources · 2019Review
- Multiple approaches to assess the safety of artisanal marine food in a tropical estuary.Environmental monitoring and assessment · 2017Article
- Performance of pyrosequencing versus MALDI-TOF MS in bacteria identification in chronic lung disease.Journal of biological methods · 2016Article
- Streptococcus gallolyticus subsp. pasteurianus infection in twin infants.Journal of clinical microbiology · 2015Article
- Metagenomics: tools and insights for analyzing next-generation sequencing data derived from biodiversity studies.Bioinformatics and biology insights · 2015Review
- Assessing the accuracy of quantitative molecular microbial profiling.International journal of molecular sciences · 2014Article
- Dokdonella koreensis bacteremia: A case report and review of the literature.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- Research on neonatal microbiomes: what neonatologists need to know.Neonatology · 2014Review
- Identification of Lactobacillus strains with probiotic features from the bottlenose dolphin (Tursiops truncatus).Journal of applied microbiology · 2013Article
- Architectural design influences the diversity and structure of the built environment microbiome.The ISME journal · 2012Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Characterization of nontypeable and atypical Streptococcus pneumoniae pediatric isolates from 1994 to 2010.Journal of clinical microbiology · 2012Article
- Pyrosequencing as a tool for better understanding of human microbiomes.Journal of oral microbiology · 2012Article
- A universal method for the identification of bacteria based on general PCR primers.Indian journal of microbiology · 2011Article
- Expecting the unexpected: nucleic acid-based diagnosis and discovery of emerging viruses.Expert review of molecular diagnostics · 2011Article
- Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis.BMC microbiology · 2011Article
- Rapid stool-based diagnosis of Clostridium difficile infection by real-time PCR in a children's hospital.Journal of clinical microbiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Sole reliance on biochemical methods can limit the clinical microbiology laboratory's ability to identify bacterial pathogens. This study describes the incorporation of DNA pyrosequencing-based identification for routine pathogen identification of atypical clinical isolates in a large children's hospital. The assay capitalized on the highly conserved nature of 16S rRNA genes by positioning amplification and sequencing primers in conserved target sequences flanking the variable V1 and V3 regions. A total of 414 isolates of 312 pediatric patients were tested by DNA pyrosequencing during the time period from December 2003 to July 2006. Seventy-eight different genera were specified by DNA pyrosequencing, and isolates were derived from diverse specimen types. By integrating DNA sequencing of bacterial pathogens with conventional microbiologic methods, isolates that lacked a definitive identification by biochemical testing yielded genus- or species-level identifications in approximately 90% of cases by pyrosequencing. Improvements incorporated into the assay process during the period of clinical testing included software enhancements, improvements in sequencing reagents, and refinements in database search strategies. Coupled with isolation by bacteriologic culture and biochemical testing, DNA pyrosequencing-based bacterial identification was a valuable tool that markedly improved bacterial pathogen identification in a pediatric hospital setting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.