ArticleJournal of virology2007
Association of the astrovirus structural protein VP90 with membranes plays a role in virus morphogenesis.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
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- Porcine astrovirus: a review.Archives of virology · 2026Review
- Distinct intracellular and extracellular maturation of goose astrovirus 2 capsid protein and the potential of virus-like particles as a novel subunit vaccine.Frontiers in veterinary science · 2026Article
- Epidemiological investigation and pathogenicity analysis of waterfowl astroviruses in some areas of China.Frontiers in microbiology · 2024Article
- Astrovirus replication is dependent on induction of double-membrane vesicles through a PI3K-dependent, LC3-independent pathway.Journal of virology · 2023Article
- Human astrovirus capsid protein releases a membrane lytic peptide upon trypsin maturation.Journal of virology · 2023Article
- The Association of Human Astrovirus with Extracellular Vesicles Facilitates Cell Infection and Protects the Virus from Neutralizing Antibodies.Journal of virology · 2022Article
- A Review of Emerging Goose Astrovirus Causing Gout.BioMed research international · 2022Review
- Detection of mink astrovirus in Poland and further phylogenetic comparison with other European and Canadian astroviruses.Virus genes · 2021Article
- Structural Insights into the Human Astrovirus Capsid.Viruses · 2021Review
- Isolation and Characterization of Porcine Astrovirus 5 from a Classical Swine Fever Virus-Infected Specimen.Journal of virology · 2020Article
- OASL Triggered by Novel Goose Astrovirus via ORF2 Restricts Its Replication.Journal of virology · 2020Article
- A molecular survey, whole genome sequencing and phylogenetic analysis of astroviruses from roe deer.BMC veterinary research · 2020Article
- Article
- Detection and phylogenetic characterization of astroviruses in insectivorous bats from Central-Southern Italy.Zoonoses and public health · 2018Article
- The Ubiquitin-Proteasome System Is Necessary for Efficient Replication of Human Astrovirus.Journal of virology · 2018Article
- Viral hijacking of host caspases: an emerging category of pathogen-host interactions.Cell death and differentiation · 2017Review
- Complete genome sequence analysis of chicken astrovirus isolate from India.Veterinary research communications · 2017Article
- The Astrovirus Capsid: A Review.Viruses · 2017Review
- Structure of a Human Astrovirus Capsid-Antibody Complex and Mechanistic Insights into Virus Neutralization.Journal of virology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
VP90, the capsid polyprotein precursor of human astrovirus Yuc8, is assembled into viral particles, and its processing at the carboxy terminus by cellular caspases, to yield VP70, has been correlated with the cell release of the virus. Here, we characterized the effect of the VP90-VP70 processing on the properties of these proteins, as well as on their intracellular distribution. VP90 was found in membrane-enriched fractions (mVP90), as well as in fractions enriched in cytosolic proteins (cVP90), while VP70 was found exclusively in the latter fractions. Upon trypsin activation, infectivity was detected in all VP90-containing fractions, confirming that both mVP90 and cVP90 are able to assemble into particles; however, the two forms of VP90 showed differential sensitivities to trypsin, especially at their carboxy termini, which in the case of mVP90 was shown to remain membrane associated after protease digestion. Structural protein oligomers were detected in purified VP70-containing viruses, as well as in membrane-enriched fractions, but they were less evident in cytosolic fractions. Ultrastructural studies of infected cells revealed different types of viral particles, some of which appeared to be associated with membranes. By immunoelectron microscopy, structural proteins were shown to form virus particles in clusters and to associate with the edges of vesicles induced during infection, which also appear to contain subviral particles inside. Nonstructural proteins and viral RNA colocalized with mVP90, but not with cVP90, suggesting that mVP90 might represent the form of the protein that is initially assembled into particles, at the sites where the virus genome is being replicated.
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Registered trials
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