ArticleNucleic acids research2007
Ionizing radiation and restriction enzymes induce microhomology-mediated illegitimate recombination in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Review
- Mitochondrial genome plasticity of mammalian species.BMC genomics · 2024Article
- Aerospace Technology Improves Fermentation Potential of Microorganisms.Frontiers in microbiology · 2022Review
- Advances in space microbiology.iScience · 2021Review
- Rapid functional activation of a horizontally transferred eukaryotic gene in a bacterial genome in the absence of selection.Nucleic acids research · 2019Article
- Numtogenesis as a mechanism for development of cancer.Seminars in cancer biology · 2017Review
- Requirement of POL3 and POL4 on non-homologous and microhomology-mediated end joining in rad50/xrs2 mutants of Saccharomyces cerevisiae.Mutagenesis · 2015Article
- Mechanism of random integration of foreign DNA in transgenic mice.Transgenic research · 2013Article
- Breakpoint characterization of a novel large intragenic deletion of MUTYH detected in a MAP patient: case report.BMC medical genetics · 2011Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- Genomic characterization of large rearrangements of the LDLR gene in Czech patients with familial hypercholesterolemia.BMC medical genetics · 2010Article
- Expanding CEP290 mutational spectrum in ciliopathies.American journal of medical genetics. Part A · 2009Article
- Rad1, rad10 and rad52 mutations reduce the increase of microhomology length during radiation-induced microhomology-mediated illegitimate recombination in saccharomyces cerevisiae.Radiation research · 2009Article
- Ends-in vs. ends-out targeted insertion mutagenesis in Saccharomyces castellii.Current genetics · 2009Article
- Germline CDH1 deletions in hereditary diffuse gastric cancer families.Human molecular genetics · 2009Article
- Ionizing radiation induces microhomology-mediated end joining in trans in yeast and mammalian cells.Radiation research · 2009Article
- Ionizing radiation: how fungi cope, adapt, and exploit with the help of melanin.Current opinion in microbiology · 2008Review
- MMEJ repair of double-strand breaks (director's cut): deleted sequences and alternative endings.Trends in genetics : TIG · 2008Review
- Small deletion variants have stable breakpoints commonly associated with alu elements.PloS one · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
DNA double-strand breaks can be repaired by illegitimate recombination without extended sequence homology. A distinct mechanism namely microhomology-mediated recombination occurs between a few basepairs of homology that is associated with deletions. Ionizing radiation and restriction enzymes have been shown to increase the frequency of nonhomologous integration in yeast. However, the mechanism of such enhanced recombination events is not known. Here, we report that both ionizing radiation and restriction enzymes increase the frequency of microhomology-mediated integration. Irradiated yeast cells displayed 77% microhomology-mediated integration, compared to 27% in unirradiated cells. Radiation-induced integration exhibited lack of deletions at genomic insertion sites, implying that such events are likely to occur at undamaged sites. Restriction enzymes also enhanced integration events at random non-restriction sites via microhomology-mediated recombination. Furthermore, generation of a site-specific I-SceI-mediated double-strand break induces microhomology-mediated integration randomly throughout the genome. Taken together, these results suggest that double-strand breaks induce a genome-wide microhomology-mediated illegitimate recombination pathway that facilitates integration probably in trans at non-targeted sites and might be involved in generation of large deletions and other genomic rearrangements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.