ArticleAntimicrobial agents and chemotherapy2007
Multiplex asymmetric PCR-based oligonucleotide microarray for detection of drug resistance genes containing single mutations in Enterobacteriaceae.
Article in Antimicrobial agents and chemotherapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Characterization of phenotypic and genotypic traits of Klebsiella pneumoniae strains resistant to 3rd generation cephalosporins in hospital settings: A case study in Ho Chi Minh City, Vietnam.Molecular biology reports · 2025Article
- Antibiotic Resistance Mediated byAntibiotics (Basel, Switzerland) · 2023Article
- A novel assay based on DNA melting temperature for multiplexed identification of SARS-CoV-2 and influenza A/B viruses.Frontiers in microbiology · 2023Article
- Article
- Prevalence of ESBL and AmpC genes inNew microbes and new infections · 2022Article
- An advanced and efficient asymmetric PCR method for microarray applications.Frontiers in bioengineering and biotechnology · 2022Article
- Multiplexed microRNA Expression Profiling by Combined Asymmetric PCR and Label-Free Detection using Silicon Photonic Sensor Arrays.Analytical methods : advancing methods and applications · 2018Article
- TheInternational journal of genomics · 2018Article
- Article
- Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array.BMC research notes · 2014Article
- Universal Probe Library based real-time PCR for rapid detection of bacterial pathogens from positive blood culture bottles.World journal of microbiology & biotechnology · 2014Article
- Methods for the preparation of large quantities of complex single-stranded oligonucleotide libraries.PloS one · 2014Article
- Single-stranded DNA catalyzes hybridization of PCR-products to microarray capture probes.PloS one · 2014Article
- Article
- Performance assessment of the CapitalBio mycobacterium identification array system for identification of mycobacteria.Journal of clinical microbiology · 2012Article
- Article
- Development and comparison of two assay formats for parallel detection of four biothreat pathogens by using suspension microarrays.PloS one · 2012Article
- Article
- Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.Journal of clinical microbiology · 2010Article
- Evaluation of a commercial microarray system for detection of SHV-, TEM-, CTX-M-, and KPC-type beta-lactamase genes in Gram-negative isolates.Journal of clinical microbiology · 2010Article
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9 authors.
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Abstract
A multiplex asymmetric PCR (MAPCR)-based microarray method was developed for the detection of 10 known extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamase genes in gram-negative bacteria and for the typing of six important point mutations (amino acid positions 35, 43, 130, 179, 238, and 240) in the bla(SHV) gene. The MAPCR is based on a two-round reaction to promote the accumulation of the single-stranded amplicons amenable for microarray hybridization by employing multiple universal unrelated sequence-tagged primers and elevating the annealing temperature at the second round of amplification. A strategy to improve the discrimination efficiency of the microarray was constituted by introducing an artificial mismatch into some of the allele-specific oligonucleotide probes. The microarray assay correctly identified the resistance genes in both the reference strains and some 111 clinical isolates, and these results were also confirmed for some isolates by direct DNA sequence analysis. The resistance genotypes determined by the microarray correlated closely with phenotypic MIC susceptibility testing. This fast MAPCR-based microarray method should prove useful for undertaking important epidemiological studies concerning ESBLs and plasmid-mediated AmpC enzymes and could also prove invaluable as a preliminary screen to supplement phenotypic testing for clinical diagnostics.
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