ArticleTraffic (Copenhagen, Denmark)2007
Targeting of Sna3p to the endosomal pathway depends on its interaction with Rsp5p and multivesicular body sorting on its ubiquitylation.
Article in Traffic (Copenhagen, Denmark), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- The Dsc ubiquitin ligase complex identifies transmembrane degrons to degrade orphaned proteins at the Golgi.Nature communications · 2024Article
- Nitrogen coordinated import and export of arginine across the yeast vacuolar membrane.PLoS genetics · 2020Article
- Subcellular Journey of Rare Cold Inducible 2 Protein in Plant Under Stressful Condition.Frontiers in plant science · 2020Review
- Analysis of ubiquitin recognition by the HECT ligase E6AP provides insight into its linkage specificity.The Journal of biological chemistry · 2019Article
- Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins.BMC biology · 2018Article
- Mechanism of ubiquitin chain synthesis employed by a HECT domain ubiquitin ligase.The Journal of biological chemistry · 2017Article
- Structural insights into the catalysis and regulation of E3 ubiquitin ligases.Nature reviews. Molecular cell biology · 2016Review
- A family of tetraspans organizes cargo for sorting into multivesicular bodies.Developmental cell · 2015Article
- Membrane trafficking in the yeast Saccharomyces cerevisiae model.International journal of molecular sciences · 2015Review
- Review
- Lysine-targeting specificity in ubiquitin and ubiquitin-like modification pathways.Nature structural & molecular biology · 2014Review
- Article
- Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast.The Journal of biological chemistry · 2013Article
- The ubiquitin-proteasome system regulates mitochondrial intermembrane space proteins.Molecular and cellular biology · 2013Article
- Article
- A dual role for K63-linked ubiquitin chains in multivesicular body biogenesis and cargo sorting.Molecular biology of the cell · 2012Article
- Sna3 is an Rsp5 adaptor protein that relies on ubiquitination for its MVB sorting.Traffic (Copenhagen, Denmark) · 2012Article
- Rsp5 ubiquitin ligase is required for protein trafficking in Saccharomyces cerevisiae COPI mutants.PloS one · 2012Article
- Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals.European journal of cell biology · 2011Article
- A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.Molecular & cellular proteomics : MCP · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rsp5p is an ubiquitin (Ub)-protein ligase of the Nedd4 family that carries WW domains involved in interaction with PPXY-containing proteins. It plays a key role at several stages of intracellular trafficking, such as Ub-mediated internalization of endocytic cargoes and Ub-mediated sorting of membrane proteins to internal vesicles of multivesicular bodies (MVBs), a process that is crucial for their subsequent targeting to the vacuolar lumen. Sna3p is a membrane protein previously described as an Ub-independent MVB cargo, but proteomic studies have since shown it to be an ubiquitylated protein. Sna3p carries a PPXY motif. We observed that this motif mediates its interaction with Rsp5p WW domains. Mutation of either the Sna3p PPXY motif or the Rsp5p WW3 domain or reduction in the amounts of Rsp5 results in the mistargeting of Sna3p to multiple mobile vesicles and prevents its sorting to the endosomal pathway. This sorting defect appears to occur prior to the defect displayed in rsp5 mutants by other MVB cargoes, which are correctly sorted to the endosomal pathway but missorted to the vacuolar membrane instead of the vacuolar lumen. Sna3p is polyubiquitylated on one target lysine, and a mutant Sna3p lacking its target lysine displays defective MVB sorting. Sna3p undergoes Rsp5-dependent polyubiquitylation, with K63-linked Ub chains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.