ArticleApplied and environmental microbiology2007
Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Review
- Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample.Scientific reports · 2021Article
- Distribution and diversity of nitrite-dependent anaerobic methane-oxidising bacteria in the sediments of the Qiantang River.Microbial ecology · 2014Article
- Allelic sequence heterozygosity in single Giardia parasites.BMC microbiology · 2012Article
- Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples.Applied and environmental microbiology · 2010Article
- Detection of Batrachochytrium dendrobatidis in endemic salamander species from central Texas.EcoHealth · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.
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Registered trials
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