ArticleJournal of bacteriology2007
Specificity and regulation of interaction between the PII and AmtB1 proteins in Rhodospirillum rubrum.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Function, Structure, and Regulation of Nitrogen Fixation-like Metalloproteins for Nitrogen, Energy, Carbon, and Sulfur Metabolism.Chemical reviews · 2026Review
- A singular PpaA/AerR-like protein inmSystems · 2023Article
- Potential of Phototrophic Purple Nonsulfur Bacteria to Fix Nitrogen in Rice Fields.Microorganisms · 2021Review
- Identification and functional analysis of glutamine transporter inJournal of oral microbiology · 2020Article
- A Quinoline-Appended Cyclodextrin Derivative as a Highly Selective Receptor and Colorimetric Probe for Nucleotides.iScience · 2020Article
- Posttranslational modification of dinitrogenase reductase in Rhodospirillum rubrum treated with fluoroacetate.World journal of microbiology & biotechnology · 2018Article
- Energy shifts induce membrane sequestration of DraG in Rhodospirillum rubrum independent of the ammonium transporters and diazotrophic conditions.FEMS microbiology letters · 2018Article
- Identification and functional analysis of an ammonium transporter in Streptococcus mutans.PloS one · 2014Article
- P(II) signal transduction proteins are ATPases whose activity is regulated by 2-oxoglutarate.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Complete genome sequence of Rhodospirillum rubrum type strain (S1).Standards in genomic sciences · 2011Article
- Control of AmtB-GlnK complex formation by intracellular levels of ATP, ADP, and 2-oxoglutarate.The Journal of biological chemistry · 2010Article
- Nitrogenase switch-off and regulation of ammonium assimilation in response to light deprivation in Rhodospirillum rubrum are influenced by the nitrogen source used during growth.Journal of bacteriology · 2010Article
- Effect of perturbation of ATP level on the activity and regulation of nitrogenase in Rhodospirillum rubrum.Journal of bacteriology · 2009Article
- In vitro interactions between the PII proteins and the nitrogenase regulatory enzymes dinitrogenase reductase ADP-ribosyltransferase (DraT) and dinitrogenase reductase-activating glycohydrolase (DraG) in Azospirillum brasilense.The Journal of biological chemistry · 2009Article
- A genetically encoded fluorescent reporter of ATP:ADP ratio.Nature methods · 2009Article
- Identification and functional characterization of NifA variants that are independent of GlnB activation in the photosynthetic bacterium Rhodospirillum rubrum.Microbiology (Reading, England) · 2008Article
- Ammonia-induced formation of an AmtB-GlnK complex is not sufficient for nitrogenase regulation in the photosynthetic bacterium Rhodobacter capsulatus.Journal of bacteriology · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The nitrogen regulatory protein P(II) and the ammonia gas channel AmtB are both found in most prokaryotes. Interaction between these two proteins has been observed in several organisms and may regulate the activities of both proteins. The regulation of their interaction is only partially understood, and we show that in Rhodospirillum rubrum one P(II) homolog, GlnJ, has higher affinity for an AmtB(1)-containing membrane than the other two P(II) homologs, GlnB and GlnK. This interaction strongly favors the nonuridylylated form of GlnJ and is disrupted by high levels of 2-ketoglutarate (2-KG) in the absence of ATP or low levels of 2-KG in the presence of ATP. ADP inhibits the destabilization of the GlnJ-AmtB(1) complex in the presence of ATP and 2-KG, supporting a role for P(II) as an energy sensor measuring the ratio of ATP to ADP. In the presence of saturating levels of ATP, the estimated K(d) of 2-KG for GlnJ bound to AmtB(1) is 340 microM, which is higher than that required for uridylylation of GlnJ in vitro, about 5 microM. This supports a model where multiple 2-KG and ATP molecules must bind a P(II) trimer to stimulate release of P(II) from AmtB(1), in contrast to the lower 2-KG requirement for productive uridylylation of P(II) by GlnD.
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