ArticleJournal of bacteriology2007
The unusual 23S rRNA gene of Coxiella burnetii: two self-splicing group I introns flank a 34-base-pair exon, and one element lacks the canonical omegaG.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
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- Horizontally Acquired Biosynthesis Genes BoostFrontiers in cellular and infection microbiology · 2017Article
- Genome sequence of Coxiella burnetii strain Namibia.Standards in genomic sciences · 2014Article
- Multiple self-splicing introns in the 16S rRNA genes of giant sulfur bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Genetics of Coxiella burnetii: on the path of specialization.Future microbiology · 2011Review
- Ribozyme stability, exon skipping, and a potential role for RNA helicase in group I intron splicing by Coxiella burnetii.Journal of bacteriology · 2011Article
- Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.PloS one · 2010Article
- Pentamidine inhibits Coxiella burnetii growth and 23S rRNA intron splicing in vitro.International journal of antimicrobial agents · 2010Article
- Group I introns and inteins: disparate origins but convergent parasitic strategies.Journal of bacteriology · 2009Review
- A unique group I intron in Coxiella burnetii is a natural splice mutant.Journal of bacteriology · 2009Article
- Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella.Infection and immunity · 2009Article
- Toxic introns and parasitic intein in Coxiella burnetii: legacies of a promiscuous past.Journal of bacteriology · 2008Article
- Sustained axenic metabolic activity by the obligate intracellular bacterium Coxiella burnetii.Journal of bacteriology · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We describe the presence and characteristics of two self-splicing group I introns in the sole 23S rRNA gene of Coxiella burnetii. The two group I introns, Cbu.L1917 and Cbu.L1951, are inserted at sites 1917 and 1951 (Escherichia coli numbering), respectively, in the 23S rRNA gene of C. burnetii. Both introns were found to be self-splicing in vivo and in vitro even though the terminal nucleotide of Cbu.L1917 is adenine and not the canonical conserved guanine, termed OmegaG, found in Cbu.L1951 and all other group I introns described to date. Predicted secondary structures for both introns were constructed and revealed that Cbu.L1917 and Cbu.L1951 were group IB2 and group IA3 introns, respectively. We analyzed strains belonging to eight genomic groups of C. burnetii to determine sequence variation and the presence or absence of the elements and found both introns to be highly conserved (>/=99%) among them. Although phylogenetic analysis did not identify the specific identities of donors, it indicates that the introns were likely acquired independently; Cbu.L1917 was acquired from other bacteria like Thermotoga subterranea and Cbu.L1951 from lower eukaryotes like Acanthamoeba castellanii. We also confirmed the fragmented nature of mature 23S rRNA in C. burnetii due to the presence of an intervening sequence. The presence of three selfish elements in C. burnetii's 23S rRNA gene is very unusual for an obligate intracellular bacterium and suggests a recent shift to its current lifestyle from a previous niche with greater opportunities for lateral gene transfer.
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