Trial reportAmerican journal of physiology. Heart and circulatory physiology2007
NFKB1 promoter variation implicates shear-induced NOS3 gene expression and endothelial function in prehypertensives and stage I hypertensives.
Trial report in American journal of physiology. Heart and circulatory physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Significant association between rs28362491 polymorphism in NF-κB1 gene and coronary artery disease: a meta-analysis.BMC cardiovascular disorders · 2020Pooled it
- Synergistic effects of NF-κB1 and inflammatory pathway polymorphisms on hypertension and dyslipidemia susceptibility in type 2 diabetes.Diabetology & metabolic syndrome · 2025Article
- Interaction of dietary patterns with rs28362491 on severity of coronary artery stenosis in patients undergoing coronary angiography.Scientific reports · 2023Article
- Association of NFKB1 gene polymorphism (rs28362491) with cardiometabolic risk factor in patients undergoing coronary angiography.Journal of cardiovascular and thoracic research · 2023Article
- Association of NFKB1 gene rs28362491 mutation with the occurrence of major adverse cardiovascular events.BMC cardiovascular disorders · 2022Article
- Intracranial Aneurysms Induced byFrontiers in neurology · 2022Article
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- Article
- The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells.Mediators of inflammation · 2021Article
- A Systematic Review Examining the Approaches Used to Estimate Interindividual Differences in Trainability and Classify Individual Responses to Exercise Training.Frontiers in physiology · 2021Review
- NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population.Scientific reports · 2020Article
- NFKB1 gene rs28362491 polymorphism is associated with the susceptibility of acute coronary syndrome.Bioscience reports · 2019Article
- Effects of resveratrol on laminar shear stress-induced mitochondrial biogenesis in human vascular endothelial cells.Journal of exercise nutrition & biochemistry · 2019Article
- Association of NFKB1A and microRNAs variations and the susceptibility to atherosclerosis.Journal of genetics · 2017Article
- A genome-wide association study of essential hypertension in an Australian population using a DNA pooling approach.Molecular genetics and genomics : MGG · 2017Article
- Genetic Regulation of Endothelial Vasomotor Function.Frontiers in physiology · 2016Review
- Shear stress-induced mitochondrial biogenesis decreases the release of microparticles from endothelial cells.American journal of physiology. Heart and circulatory physiology · 2015Article
- Article
- Extracellular HSP27 acts as a signaling molecule to activate NF-κB in macrophages.Cell stress & chaperones · 2013Article
- S-nitrosylation in the regulation of gene transcription.Biochimica et biophysica acta · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
In endothelial cells, NF-kappaB is an important intracellular signaling molecule by which changes in wall shear stress are transduced into the nucleus to initiate downstream endothelial nitric oxide synthase (NOS3) gene expression. We investigated whether NF-kappa light-chain gene enhancer in B cells 1 (NFKB1) promoter polymorphism ((-94)NFKB1 I/D, where I is the insertion allele and D is the deletion allele) was associated with 1) NOS3 gene expression in endothelial cells under physiological levels of unidirectional laminar shear stress (LSS) and 2) endothelial function in prehypertensive and stage I hypertensive individuals before and after a 6-mo supervised endurance exercise intervention. Competitive EMSAs revealed that proteins present in the nuclei of endothelial cells preferentially bound to the I allele NFKB1 promoter compared with the D allele. Reporter gene assays showed that the I allele promoter had significantly higher activity than the D allele. In agreement with these observations, homozygous II genotype cells had higher p50 expression levels than homozygous DD genotype cells. Cells with the homozygous II genotype showed a greater increase in NOS3 protein expression than did homozygous DD genotype cells under LSS. Functional experiments on volunteers confirmed higher baseline reactive hyperemic forearm blood flow, and, furthermore, the subgroup analysis revealed that DD homozygotes were significantly less prevalent in the exercise responder group compared with II and ID genotypes. We conclude that the (-94)NFKB1 I/D promoter variation contributes to the modulation of vascular function and adaptability to exercise-induced flow shear stress, most likely due to differences in NFKB1 gene transactivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.