ArticleNature cell biology2007
Exocytotic catecholamine release is not associated with cation flux through channels in the vesicle membrane but Na+ influx through the fusion pore.
Article in Nature cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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Who cites it
38 citing papers in PubMed.
- Chromogranin A mediates glucocorticoid-induced enhancement of vesicular dopamine storage and secretion in PC12 cells.The Journal of biological chemistry · 2026Article
- α-Synuclein oligomers slow down action potential firing and enhance dopamine release by increasing Cav2.2 currents in midbrain dopaminergic neurons.The Journal of physiology · 2026Article
- Fusion Pores as Regulators of Quantal Size and Cellular Physiology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Using multiscale molecular dynamics simulations to explore the fusion machinery underlying neurotransmitter release.Quarterly reviews of biophysics · 2025Review
- All SNAP25 molecules in the vesicle-plasma membrane contact zone change conformation during vesicle priming.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.Nature communications · 2023Article
- Patch Amperometry and Intracellular Patch Electrochemistry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Illuminating membrane structural dynamics of fusion and endocytosis with advanced light imaging techniques.Biochemical Society transactions · 2022Review
- Neuroligin-3 confines AMPA receptors into nanoclusters, thereby controlling synaptic strength at the calyx of Held synapses.Science advances · 2022Article
- TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis.eLife · 2021Article
- Fusion pores with low conductance are cation selective.Cell reports · 2021Article
- Fusion Pore Expansion and Contraction during Catecholamine Release from Endocrine Cells.Biophysical journal · 2020Article
- The receptor tyrosine kinase EPHB6 regulates catecholamine exocytosis in adrenal gland chromaffin cells.The Journal of biological chemistry · 2020Article
- Phosphatidylserine is critical for vesicle fission during clathrin-mediated endocytosis.Journal of neurochemistry · 2020Article
- Extracellular and intracellular sphingosine-1-phosphate distinctly regulates exocytosis in chromaffin cells.Journal of neurochemistry · 2019Article
- Synaptobrevin-2 C-Terminal Flexible Region Regulates the Discharge of Catecholamine Molecules.Biophysical journal · 2019Article
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- The Transmembrane Domain of Synaptobrevin Influences Neurotransmitter Flux through Synaptic Fusion Pores.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements.Pflugers Archiv : European journal of physiology · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Release of charged neurotransmitter molecules through a narrow fusion pore requires charge compensation by other ions. It has been proposed that this may occur by ion flow from the cytosol through channels in the vesicle membrane, which would generate a net outward current. This hypothesis was tested in chromaffin cells using cell-attached patch amperometry that simultaneously measured catecholamine release from single vesicles and ionic current across the patch membrane. No detectable current was associated with catecholamine release indicating that <2% of cations, if any, enter the vesicle through its membrane. Instead, we show that flux of catecholamines through the fusion pore, measured as an amperometric foot signal, decreases when the extracellular cation concentration is reduced. The results reveal that the rate of transmitter release through the fusion pore is coupled to net Na+ influx through the fusion pore, as predicted by electrodiffusion theory applied to fusion-pore permeation, and suggest a prefusion rather than postfusion role for vesicular cation channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.