ArticleReproduction (Cambridge, England)2007
Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function.
Article in Reproduction (Cambridge, England), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 97 citations in OpenAlex.
- Cross-species oncolytic virotherapy in vitro: BoHV4EGFPΔTK as a novel candidate against canine osteosarcoma and histiocytic sarcoma.Veterinary research communications · 2026Article
- Distal and local mucosal immunization with a BoHV-4-based vector delivering CpHV-1 gD confers protection against intravaginal CpHV-1 challenge in goats.Frontiers in immunology · 2026Article
- Article
- Microbial Gatekeepers of Fertility in the Female Reproductive Microbiome of Cattle.International journal of molecular sciences · 2024Review
- First detection and impact of bovine herpesvirus type 4 on dairy cattle reproduction in Thailand.Veterinary world · 2024Article
- Lessons learned from bovine subclinical endometritis: A systematic review exploring its potential relevance to chronic endometritis in women.Reproduction & fertility · 2024Article
- Article
- Unveiling the Role of Bovine Herpesvirus Type 4 (BHV-4) in Dairy Cow Reproductive Disorders: Insights From a Multifaceted Study in China.Transboundary and emerging diseases · 2024Article
- The curative effect of ozonated bidistilled water on Escherichia coli-induced endometritis in rats.Veterinary medicine and science · 2023Article
- Role of Genital Tract Bacteria in Promoting Endometrial Health in Cattle.Microorganisms · 2022Article
- Review
- Modulation of Bovine Endometrial Cell Receptors and Signaling Pathways as a Nanotherapeutic Exploration against Dairy Cow Postpartum Endometritis.Animals : an open access journal from MDPI · 2021Review
- Gene expression and in vitro replication of bovine gammaherpesvirus type 4.Archives of virology · 2021Article
- Assessment of replication of bovine herpesvirus type 4 in human glioblastoma and breast cancer cells as a potential oncolytic virus.Virus genes · 2021Article
- An outbreak of Neospora caninum abortion in a dairy herd from the State of Georgia, United States.Veterinary medicine and science · 2021Article
- Article
- Importance of Viral Disease in Dairy Cow Fertility.Engineering (Beijing, China) · 2020Review
- Improved filtration method to isolate pure populations of primary bovine endometrial epithelial and stromal cells for immunological studies.Veterinary research communications · 2020Article
- Unravelling the first key steps in equine herpesvirus type 5 (EHV5) pathogenesis using ex vivo and in vitro equine models.Veterinary research · 2019Article
- Gammaherpesvirus BoHV-4 infects bovine respiratory epithelial cells mainly at the basolateral side.Veterinary research · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Bovine postpartum uterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. Although the gamma-herpesvirus bovine herpesvirus 4 (BoHV-4) has been isolated from several outbreaks of metritis or abortion, the role of viruses in endometrial pathology and the mechanisms of viral infection of uterine cells are often ignored. The objectives of the present study were to explore the interaction, tropism and outcomes of BoHV-4 challenge of endometrial stromal and epithelial cells. Endometrial stromal and epithelial cells were purified and infected with a recombinant BoHV-4 carrying an enhanced green fluorescent protein (EGFP) expression cassette to monitor the establishment of infection. BoHV-4 efficiently infected both stromal and epithelial cells, causing a strong non-apoptotic cytopathic effect, associated with robust viral replication. The crucial step for the BoHV-4 endometriotropism appeared to be after viral entry as there was enhanced transactivation of the BoHV-4 immediate early 2 gene promoter following transient transfection into the endometrial cells. Infection with BoHV-4 increased cyclooxygenase 2 protein expression and prostaglandin estradiol secretion in endometrial stromal cells, but not epithelial cells. Bovine macrophages are persistently infected with BoHV-4, and co-culture with endometrial stromal cells reactivated BoHV-4 replication in the persistently infected macrophages, suggesting a symbiotic relationship between the cells and virus. In conclusion, the present study provides evidence of cellular and molecular mechanisms, supporting the concept that BoHV-4 is a pathogen associated with uterine disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.