ArticleJournal of immunology (Baltimore, Md. : 1950)2007
LFA-1-mediated T cell costimulation through increased localization of TCR/class II complexes to the central supramolecular activation cluster and exclusion of CD45 from the immunological synapse.
Article in Journal of immunology (Baltimore, Md. : 1950), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 62 citations in OpenAlex.
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- Bayesian metamodeling of early T-cell antigen receptor signaling accounts for its nanoscale activation patterns.Frontiers in immunology · 2024Article
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- Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer.Methods in cell biology · 2023Article
- Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1.JCI insight · 2022Article
- Size-dependent activation of CAR-T cells.Science immunology · 2022Article
- Structural, biochemical, and functional properties of the Rap1-Interacting Adaptor Molecule (RIAM).Biomedical journal · 2022Review
- Characterization of mechanisms positioning costimulatory complexes in immune synapses.iScience · 2021Article
- Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation.Cell reports · 2021Article
- Article
- Expression Dynamics of theFrontiers in immunology · 2021Article
- PI3K in T Cell Adhesion and Trafficking.Frontiers in immunology · 2021Review
- Increasing LFA-1 Expression Enhances Immune Synapse Architecture and T Cell Receptor Signaling in Jurkat E6.1 Cells.Frontiers in cell and developmental biology · 2021Article
- The CAR T-Cell Mechanoimmunology at a Glance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- IL-10 inducible CD8Cancer immunology, immunotherapy : CII · 2018Article
- Article
- Chimeric antigen receptor T cells form nonclassical and potent immune synapses driving rapid cytotoxicity.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Integrins in T Cell Physiology.International journal of molecular sciences · 2018Review
- Integrins Modulate T Cell Receptor Signaling by Constraining Actin Flow at the Immunological Synapse.Frontiers in immunology · 2018Article
- A novel intracellular pool of LFA-1 is critical for asymmetric CD8The Journal of cell biology · 2017Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
T cell activation is associated with a dramatic reorganization of cell surface proteins and associated signaling components into discrete subdomains within the immunological synapse in T cell:APC conjugates. However, the signals that direct the localization of these proteins and the functional significance of this organization have not been established. In this study, we have used wild-type and LFA-1-deficient, DO11.10 TCR transgenic T cells to examine the role of LFA-1 in the formation of the immunological synapse. We found that coengagement of LFA-1 is not required for the formation of the central supramolecular activation cluster (cSMAC) region, but does increase the accumulation of TCR/class II complexes within the cSMAC. In addition, LFA-1 is required for the recruitment and localization of talin into the peripheral supramolecular activation cluster region and exclusion of CD45 from the synapse. The ability of LFA-1 to increase the amount of TCR engaged during synapse formation and segregate the phosphatase, CD45, from the synapse suggests that LFA-1 might enhance proximal TCR signaling. To test this, we combined flow cytometry-based cell adhesion and calcium-signaling assays and found that coengagement of LFA-1 significantly increased the magnitude of the intracellular calcium response following Ag presentation. These data support the idea that in addition to its important role on regulating T cell:APC adhesion, coengagement of LFA-1 can enhance T cell signaling, and suggest that this may be accomplished in part through the organization of proteins within the immunological synapse.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.