ArticleProceedings of the National Academy of Sciences of the United States of America2007
Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
72 citing papers in PubMed, 1 synthesis or guideline pooled it, 137 citations in OpenAlex.
- A meta-analysis of microarray gene expression in mouse stem cells: redefining stemness.PloS one · 2008Pooled it
- The Role of Epigenetic Modifications in Enhancing Bone Marrow Transplantation Efficacy in Cancer.Advances in experimental medicine and biology · 2025Review
- Functions and regulatory mechanisms of resting hematopoietic stem cells: a promising targeted therapeutic strategy.Stem cell research & therapy · 2023Review
- Emerging Evidence of the Significance of Thioredoxin-1 in Hematopoietic Stem Cell Aging.Antioxidants (Basel, Switzerland) · 2022Review
- High-throughput enrichment and isolation of megakaryocyte progenitor cells from the mouse bone marrow.Scientific reports · 2021Article
- Mechanisms and rejuvenation strategies for aged hematopoietic stem cells.Journal of hematology & oncology · 2020Review
- Article
- The eroding chromatin landscape of aging stem cells.Translational medicine of aging · 2020Article
- Article
- PLZF limits enhancer activity during hematopoietic progenitor aging.Nucleic acids research · 2019Article
- Engineering Lineage Potency and Plasticity of Stem Cells using Epigenetic Molecules.Scientific reports · 2018Article
- GATA1 insufficiencies in primary myelofibrosis and other hematopoietic disorders: consequences for therapy.Expert review of hematology · 2018Review
- Automatic identification of informative regions with epigenomic changes associated to hematopoiesis.Nucleic acids research · 2017Article
- Chromatin dynamics during the differentiation of long-term hematopoietic stem cells to multipotent progenitors.Blood advances · 2017Article
- The Regulatory Capacity of Bivalent Genes-A Theoretical Approach.International journal of molecular sciences · 2017Article
- Article
- A comparative study of ChIP-seq sequencing library preparation methods.BMC genomics · 2016Article
- Epigenetic Control of Haematopoietic Stem Cell Aging and Its Clinical Implications.Stem cells international · 2016Review
- Epigenetic Control of Stem Cell Potential during Homeostasis, Aging, and Disease.Cell stem cell · 2015Review
- Cross talk between histone deacetylase 4 and STAT6 in the transcriptional regulation of arginase 1 during mouse dendritic cell differentiation.Molecular and cellular biology · 2015Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Hematopoietic stem cells (HSC) produce all blood cell lineages by virtue of their capacity to self-renew and differentiate into progenitors with decreasing cellular potential. Recent studies suggest that epigenetic mechanisms play an important role in controlling stem cell potency and cell fate decisions. To investigate this hypothesis in HSC, we have modified the conventional chromatin immunoprecipitation assay allowing for the analysis of 50,000 prospectively purified stem and progenitor cells. Together with bisulfite sequencing analysis, we found that methylated H3K4 and AcH3 and unmethylated CpG dinucleotides colocalize across defined regulatory regions of lineage-affiliated genes in HSC. These active epigenetic histone modifications either accumulated or were replaced by increased DNA methylation and H3K27 trimethylation in committed progenitors consistent with gene expression. We also observed bivalent histone modifications at a lymphoid-affiliated gene in HSC and downstream transit-amplifying progenitors. Together, these data support a model in which epigenetic modifications serve as an important mechanism to control HSC multipotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.